Transovarial transmission of Francisella-like endosymbionts and Anaplasma phagocytophilum variants in Dermacentor albipictus (Acari: Ixodidae).
Transovarial transmission of Francisella-like endosymbionts and Anaplasma phagocytophilum variants in Dermacentor albipictus (Acari: Ixodidae).
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DOI：
10.1603/033.046.0330
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发表时间：
2009-05
影响因子：
2.1
通讯作者：
Munderloh UG
中科院分区：
文献类型：
--
作者：
Baldridge GD;Scoles GA;Burkhardt NY;Schloeder B;Kurtti TJ;Munderloh UG
白纹革蜱（Dermacentor albipictus，Packard）是一种北美蜱，以鹿和牲畜为食。它被怀疑是牛无形体病的媒介，但其微生物植物群和媒介潜力仍被低估。我们筛选了D。采用聚合酶链反应（PCR）基因扩增和序列分析，对从明尼苏达白尾鹿（Odocoileus virginianus）采集的白刺蜱进行无形体属、埃立克体属、弗朗西斯属和立克次体属细菌检测。我们检测到无形体吞噬细胞和弗朗西斯菌样内共生体（FLEs）在若虫和成年蜱的性别，分别为45%和94%的患病率。助理嗜吞噬细胞菌和FLE通过蜱个体经卵传播至F1幼虫，效率分别为10- 40%和95- 100%。FLE经卵传播给F2幼虫，该幼虫是从在小牛身上饲养至成熟的F1幼虫蜱虫的成虫后代获得的，但A。嗜吞噬细胞菌则没有。根据PCR和组织培养接种试验，A.嗜吞噬细胞菌和FLE未传播至小牛。扩增的FLE 16 S rRNA基因序列与D. albipictus，而A.嗜吞噬细胞菌与可能的人类非致病性A.在来自威斯康星州中部的白尾鹿中检测到嗜吞噬细胞菌WI-1和WI-2变体。然而，D.白纹夜蛾A.嗜吞噬细胞A.与肩突硬蜱和白尾鹿以及人类致病性A.嗜吞噬细胞菌ha变异体与I.和白足鼠Peromyscus leucopus。A.革蜱中嗜吞噬细胞A.嗜吞噬细胞菌在自然界中可能不仅仅依赖于水平传播。
Dermacentor albipictus (Packard) is a North American tick that feeds on cervids and livestock. It is a suspected vector of anaplasmosis in cattle, but its microbial flora and vector potential remain underevaluated. We screened D. albipictus ticks collected from Minnesota white-tailed deer (Odocoileus virginianus) for bacteria of the genera Anaplasma, Ehrlichia, Francisella, and Rickettsia using polymerase chain reaction (PCR) gene amplification and sequence analyses. We detected Anaplasma phagocytophilum and Francisella-like endosymbionts (FLEs) in nymphal and adult ticks of both sexes at 45 and 94% prevalences, respectively. The A. phagocytophilum and FLEs were transovarially transmitted to F1 larvae by individual ticks at efficiencies of 10–40 and 95–100%, respectively. The FLEs were transovarially transmitted to F2 larvae obtained as progeny of adults from F1 larval ticks reared to maturity on a calf, but A. phagocytophilum were not. Based on PCR and tissue culture inoculation assays, A. phagocytophilum and FLEs were not transmitted to the calf. The amplified FLE 16S rRNA gene sequences were identical to that of an FLE detected in a D. albipictus from Texas, whereas those of the A. phagocytophilum were nearly identical to those of probable human-nonpathogenic A. phagocytophilum WI-1 and WI-2 variants detected in white-tailed deer from central Wisconsin. However, the D. albipictus A. phagocytophilum sequences differed from that of the nonpathogenic A. phagocytophilum variant-1 associated with Ixodes scapularis ticks and white-tailed deer as well as that of the human-pathogenic A. phagocytophilum ha variant associated with I. scapularis and the white-footed mouse, Peromyscus leucopus. The transovarial transmission of A. phagocytophilum variants in Dermacentor ticks suggests that maintenance of A. phagocytophilum in nature may not be solely dependent on horizontal transmission.
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DOI：
10.1073/pnas.91.21.10034
发表时间：
1994-10-11
影响因子：
11.1
作者：
BLACK, WC;PIESMAN, J
通讯作者：
PIESMAN, J
影响因子：
4.4
作者：
Baldridge, GD;Burkhardt, NY;Munderloh, UG
通讯作者：
Munderloh, UG
影响因子：
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DOI：
10.4269/ajtmh.2006.75.664
发表时间：
2006-10-01
影响因子：
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作者：
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Habbema, J. Dik F.