Amyloidogenic amyloid-β-peptide variants induce microbial agglutination and exert antimicrobial activity.
Amyloidogenic amyloid-β-peptide variants induce microbial agglutination and exert antimicrobial activity.
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DOI：
10.1038/srep32228
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发表时间：
2016-09-14
影响因子：
4.6
通讯作者：
Maler JM
中科院分区：
文献类型：
--
作者：
Spitzer P;Condic M;Herrmann M;Oberstein TJ;Scharin-Mehlmann M;Gilbert DF;Friedrich O;Grömer T;Kornhuber J;Lang R;Maler JM
淀粉样β（Aβ）肽是阿尔茨海默病患者脑内斑块的主要成分。然而，Aβ肽也可在分泌区室中检测到，外周血含有40多种不同修饰和/或N-和C-末端截短的Aβ肽的复杂混合物。近年来，Aβ肽的抗感染特性已被报道。在此，我们研究了不同长度的Aβ肽与各种细菌菌株和酵母菌白色念珠菌的相互作用。淀粉样蛋白生成肽Aβ1-42、Aβ2-42和Aβ 3 p-42与微生物表面结合，但非淀粉样蛋白生成肽Aβ1-40和Aβ2-40不与微生物表面结合。通过免疫细胞化学、扫描电子显微镜和革兰氏染色观察到，用终止于42位的Aβ肽变体（Aβx-42）处理几种细菌菌株和白色念珠菌导致形成大凝集物。与Aβx-40孵育后未检测到这些聚集体。此外，Aβx-42对所有测试的病原体都具有抗菌活性，在6 h内杀死高达80%的微生物。Aβ1-40对C.白色念珠菌。存在微生物时，Aβ1-42的凝集加速。这些数据表明，淀粉样蛋白Aβx-42变体具有抗菌活性，因此可能在免疫系统中充当抗菌肽。
Amyloid-β (Aβ) peptides are the main components of the plaques found in the brains of patients with Alzheimer’s disease. However, Aβ peptides are also detectable in secretory compartments and peripheral blood contains a complex mixture of more than 40 different modified and/or N- and C-terminally truncated Aβ peptides. Recently, anti-infective properties of Aβ peptides have been reported. Here, we investigated the interaction of Aβ peptides of different lengths with various bacterial strains and the yeast Candida albicans. The amyloidogenic peptides Aβ1-42, Aβ2-42, and Aβ3p-42 but not the non-amyloidogenic peptides Aβ1-40 and Aβ2-40 bound to microbial surfaces. As observed by immunocytochemistry, scanning electron microscopy and Gram staining, treatment of several bacterial strains and Candida albicans with Aβ peptide variants ending at position 42 (Aβx-42) caused the formation of large agglutinates. These aggregates were not detected after incubation with Aβx-40. Furthermore, Aβx-42 exerted an antimicrobial activity on all tested pathogens, killing up to 80% of microorganisms within 6 h. Aβ1-40 only had a moderate antimicrobial activity against C. albicans. Agglutination of Aβ1-42 was accelerated in the presence of microorganisms. These data demonstrate that the amyloidogenic Aβx-42 variants have antimicrobial activity and may therefore act as antimicrobial peptides in the immune system.
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影响因子：
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作者：
Gilbert, Daniel F.;Meinhof, Till;Runz, Heiko
通讯作者：
Runz, Heiko
影响因子：
4.8
作者：
Bien, Jessica;Jefferson, Tamara;Pietrzik, Claus U.
通讯作者：
Pietrzik, Claus U.
影响因子：
13.8
作者：
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通讯作者：
Gallo, RL
影响因子：
64.8
作者：
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通讯作者：
KAGAN, BL
影响因子：
4.2
作者：
McManus, Roisin M.;Higgins, Sarah C.;Lynch, Marina A.
通讯作者：
Lynch, Marina A.