Hydrocarbon productivities in different Botryococcus strains: comparative methods in product quantification.
Hydrocarbon productivities in different Botryococcus strains: comparative methods in product quantification.
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DOI：
10.1007/s10811-010-9577-8
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发表时间：
2011-08
影响因子：
3.3
通讯作者：
Melis, Anastasios
中科院分区：
文献类型：
--
作者：
Eroglu, Ela;Okada, Shigeru;Melis, Anastasios
比较了产二烯和产烃的A、B两个小种的6个不同菌株的生物量和产烃能力。生物量生产力是在规定的条件下，通过实验室中菌株生长的重量法进行评估的。通过三种不同且独立的实验方法，包括完整细胞和微菌落的密度平衡、烃抽提物的分光光度分析和洗脱烃的重量定量，测量了油气生产率。所有三种碳氢化合物定量方法对每一种被检查的菌株都产生了类似的结果。B小种微藻--布氏葡萄孢霉变种。Showa和Kawaguchi-1结构性积累的葡萄孢子烯碳氢化合物分别相当于其总生物量的30%和20%。A小种微藻巴西芽孢杆菌、山中、UTEX 2441和UTEX LB572分别积累了占其总生物量14%~13%和10%的烷烃。猪葡萄球菌(UTEX 2629)是一种形态不同的绿色微藻，其碳氢化合物积累量最低，约占其总生物量的3%。结果验证了密度平衡和分光光度分析方法在灰球菌烯类碳氢化合物定量中的有效性。这些分析进展将有助于筛选和选择布氏芽胞杆菌和其他微藻，以努力识别那些具有高碳氢化合物含量的微藻，用于商业应用。
Six different strains of the green microalgae Botryococcus belonging to the A-race or B-race, accumulating alkadiene or botryococcene hydrocarbons, respectively, were compared for biomass and hydrocarbon productivities. Biomass productivity was assessed gravimetrically upon strain growth in the laboratory under defined conditions. Hydrocarbon productivities were measured by three different and independent experimental approaches, including density equilibrium of the intact cells and micro-colonies, spectrophotometric analysis of hydrocarbon extracts, and gravimetric quantitation of eluted hydrocarbons. All three hydrocarbon-quantitation methods yielded similar results for each of the strains examined. The B-race microalgae Botryococcus braunii var. Showa and Kawaguchi-1 constitutively accumulated botryococcene hydrocarbons equivalent to 30% and 20%, respectively, of their overall biomass. The A-race microalgae Botryococcus braunii, varieties Yamanaka, UTEX 2441 and UTEX LB572 constitutively accumulated alkadiene hydrocarbons ranging from 14% to 13% and 10% of their overall biomass, respectively. Botryococcus sudeticus (UTEX 2629), a morphologically different green microalga, had the lowest hydrocarbon accumulation, equal to about 3% of its overall biomass. Results validate the density equilibrium and spectrophotometric analysis methods in the quantitation of botryococcene-type hydrocarbons. These analytical advances will serve in the screening and selection of B. braunii and of other microalgae in efforts to identify those having a high hydrocarbon content for use in commercial applications.
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影响因子：
11.4
作者：
Eroglu, Ela;Melis, Anastasios
通讯作者：
Melis, Anastasios
影响因子：
3.3
作者：
An, JY;Sim, SJ;Kim, BW
通讯作者：
Kim, BW
影响因子：
3.8
作者：
Eroglu, Ela;Melis, Anastasios
通讯作者：
Melis, Anastasios
影响因子：
3.3
作者：
Beakes, GW;Cleary, AL
通讯作者：
Cleary, AL
影响因子：
2.1
作者：
Okada, S;Tonegawa, I;Yamaguchi, K
通讯作者：
Yamaguchi, K