Age- and sex-specific fear conditioning deficits in mice lacking Pcdh10, an Autism Associated Gene.
Age- and sex-specific fear conditioning deficits in mice lacking Pcdh10, an Autism Associated Gene.
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缺乏Pcdh10（一种自闭症相关基因）的小鼠中年龄和性别特异性恐惧条件反射缺陷
DOI：
10.1016/j.nlm.2020.107364
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发表时间：
2021-03
影响因子：
2.7
通讯作者：
Abel T
中科院分区：
文献类型：
--
作者：
Ferri SL;Dow HC;Schoch H;Lee JY;Brodkin ES;Abel T
PCDH 10是与自闭症谱系障碍相关的基因。它参与丘脑皮质投射的生长和树突棘的消除。之前，我们对Pcdh 10单倍不足小鼠（Pcdh 10 +/−小鼠）进行了表征，发现了雄性特异性社交缺陷和暗相活动减退。Pcdh 10 +/−雄性表现出未成熟形态的树突棘密度增加，NMDAR表达减少，基底外侧杏仁核（BLA）的γ同步性降低。在这里，我们通过测试依赖于BLA功能的恐惧记忆来进一步表征Pcdh 10 +/−小鼠。我们使用了雄性和雌性Pcdh 10 +/−小鼠及其两个年龄段的野生型同窝仔，少年和成年，并在两种学习范式中，线索和上下文恐惧条件反射。我们发现，男性在这两个年龄和两个试验表现出恐惧条件反射缺陷，但女性只受损的提示条件下的成年人。这些数据进一步证明了Pcdh 10 +/−小鼠中BLA-related行为的雄性特异性改变，并表明这些小鼠可能是解剖雄性特异性大脑和与社会和情感行为相关的行为表型的有用模型。
PCDH10 is a gene associated with Autism Spectrum Disorder. It is involved in the growth of thalamocortical projections and dendritic spine elimination. Previously, we characterized Pcdh10 haploinsufficient mice (Pcdh10+/− mice) and found male-specific social deficits and dark phase hypoactivity. Pcdh10+/− males exhibit increased dendritic spine density of immature morphology, decreased NMDAR expression, and decreased gamma synchronization in the basolateral amygdala (BLA). Here, we further characterize Pcdh10+/− mice by testing for fear memory, which relies upon BLA function. We used both male and female Pcdh10+/− mice and their wild-type littermates at two ages, juvenile and adult, and in two learning paradigms, cued and contextual fear conditioning. We found that males at both ages and in both assays exhibited fear conditioning deficits, but females were only impaired as adults in the cued condition. These data are further evidence for male-specific alterations in BLA-related behaviors in Pcdh10+/− mice and suggest that these mice may be a useful model for dissecting male specific brain and behavioral phenotypes relevant to social and emotional behaviors.
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自闭症谱系障碍的性别差异：评论。
影响因子：
6.7
作者：
Ferri SL;Abel T;Brodkin ES
通讯作者：
Brodkin ES
影响因子：
3.3
作者：
Aoki, E;Kimura, R;Hirano, S
通讯作者：
Hirano, S
影响因子：
5.3
作者：
Blume, Shannon R.;Freedberg, Mari;Rosenkranz, J. Amiel
通讯作者：
Rosenkranz, J. Amiel
影响因子：
3
作者：
Giustino TF;Maren S
通讯作者：
Maren S
影响因子：
4.1
作者：
Brandao, Marcus L.;Coimbra, Norberto C.
通讯作者：
Coimbra, Norberto C.