A Novel and Convenient Method for the Preparation and Activation of PRP without Any Additives: Temperature Controlled PRP.
A Novel and Convenient Method for the Preparation and Activation of PRP without Any Additives: Temperature Controlled PRP.
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一种无需任何添加剂即可制备和活化 PRP 的新颖便捷方法：温控 PRP
DOI：
10.1155/2018/1761865
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发表时间：
2018
影响因子：
--
通讯作者：
Hu Z
中科院分区：
文献类型：
--
作者：
Du L;Miao Y;Li X;Shi P;Hu Z
富血小板血浆（PRP）是含有富集生长因子（GF）的自体血小板浓缩物。但是，加入外源性抗凝剂和促凝剂可能会导致临床副作用，并提高PRP的价格。在此，我们报告了一种名为温控富血小板血浆（t-PRP）的新方法，其中外源性添加剂在制备和活化过程中不需要。在低温条件（4°C）下通过两步离心过程处理人血样，以获得t-PRP，并复温至37°C以激活t-PRP。处理当代PRP（c-PRP）作为对照。在制备过程中，t-PRP显示生理pH值在7.46 - 7.48之间，血小板浓度是全血的6.58 ± 0.45倍，显著高于c-PRP（4.06倍）。同时，t-PRP也维持了血浆和PRP之间稳定的GF水平。活化后，t-PRP表现出天然纤维支架，其捕获更多的血小板和GFs，并且表现出缓慢的GFs释放和降解速率。此外，t-PRP还具有促进创面愈合的作用。t-PRP是一种新的、方便的PRP制备和活化方法，无需任何添加剂。与c-PRP相比，t-PRP在保持高质量的同时反映了更多的生理特征。
Platelet rich plasma (PRP) is a concentrate of autologous platelets which contain enrichment growth factors (GFs). However, the addition of exogenous anticoagulant and procoagulant may result in clinical side effects and raise the price of PRP. Herein, we report a novel method named temperature controlled PRP (t-PRP), in which exogenous additives are dispensable in the preparation and activation process. Human blood samples were processed by a two-step centrifugation process under hypothermic conditions (4°C) to obtain t-PRP and rewarming up to 37°C to activate t-PRP. Contemporary PRP (c-PRP) was processed as the control. t-PRP showed a physiological pH value between 7.46 and 7.48 and up to 6.58 ± 0.45-fold significantly higher platelet concentration than that of whole blood compared with c-PRP (4.06-fold) in the preparation process. Meanwhile, t-PRP also maintained a stable GF level between plasma and PRP. After activation, t-PRP demonstrated natural fiber scaffolding, which trapped more platelet and GFs, and exhibited a slow release and degradation rate of GFs. In addition, t-PRP exhibited the function of promoting wound healing. t-PRP is a novel and convenient method for the preparation and activation of PRP without any additives. Compared to c-PRP, t-PRP reflects more physiologic characteristics while maintaining high quality.
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DOI：
10.1097/shk.0000000000000082
发表时间：
2014-05
期刊：
Shock (Augusta, Ga.)
影响因子：
--
作者：
Reddoch KM;Pidcoke HF;Montgomery RK;Fedyk CG;Aden JK;Ramasubramanian AK;Cap AP
通讯作者：
Cap AP
DOI：
10.1016/j.tripleo.2005.07.012
发表时间：
2006-03-01
期刊：
ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY AND ENDODONTOLOGY
影响因子：
--
作者：
Choukroun, J;Diss, A;Dohan, DM
通讯作者：
Dohan, DM
影响因子：
3.3
作者：
Cabaro, S.;D'Esposito, V.;Riccitiello, F.
通讯作者：
Riccitiello, F.
影响因子：
4.1
作者：
Masada, T;Hua, Y;Keep, RF
通讯作者：
Keep, RF
影响因子：
2.9
作者：
Baimukanova, Gyulnar;Miyazawa, Byron;Pati, Shibani
通讯作者：
Pati, Shibani