Post-genomic analyses of fungal lignocellulosic biomass degradation reveal the unexpected potential of the plant pathogen Ustilago maydis.
Post-genomic analyses of fungal lignocellulosic biomass degradation reveal the unexpected potential of the plant pathogen Ustilago maydis.
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DOI：
10.1186/1471-2164-13-57
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发表时间：
2012-02-02
期刊：
影响因子：
4.4
通讯作者：
Berrin JG
中科院分区：
文献类型：
--
作者：
Couturier M;Navarro D;Olivé C;Chevret D;Haon M;Favel A;Lesage-Meessen L;Henrissat B;Coutinho PM;Berrin JG
丝状真菌是有效的生物质降解剂，因为它们能够在富含Ligno(半)纤维素的环境中茁壮成长。在过去的十年中，真菌基因组测序计划已经产生了关于可能与木质纤维素降解有关的基因的丰富信息。目前，更多的实验研究对于深入了解参与木质纤维素降解的真菌分泌的酶池是必不可少的。在这项研究中，我们对20种丝状真菌进行了广泛的分析，这些真菌的基因组数据可以用来研究它们的生物量水解潜力。利用酶活性图谱对真菌基因组和分泌组进行比较，发现专用于植物细胞壁的碳水化合物活性酶(CAZymes)组存在差异。研究了各分泌体对麦草糖化的贡献，结果表明，大多数分泌体单独添加工业里氏木霉CL847酶鸡尾酒。出乎意料的是，植物病原体玉米黑粉菌的效果最显著，它使总糖的释放增加了57%，葡萄糖的释放增加了22%。对表现最好的分泌体的蛋白质组学分析表明，玉米单胞菌有一种特殊的酶机制，可能涉及氧化还原酶和半纤维素酶。这项研究提供了丝状真菌降解木质纤维素的机理，并允许鉴定一些酶，这些酶可能有助于进一步改进工业木质纤维素的生物转化过程。
Filamentous fungi are potent biomass degraders due to their ability to thrive in ligno(hemi)cellulose-rich environments. During the last decade, fungal genome sequencing initiatives have yielded abundant information on the genes that are putatively involved in lignocellulose degradation. At present, additional experimental studies are essential to provide insights into the fungal secreted enzymatic pools involved in lignocellulose degradation. In this study, we performed a wide analysis of 20 filamentous fungi for which genomic data are available to investigate their biomass-hydrolysis potential. A comparison of fungal genomes and secretomes using enzyme activity profiling revealed discrepancies in carbohydrate active enzymes (CAZymes) sets dedicated to plant cell wall. Investigation of the contribution made by each secretome to the saccharification of wheat straw demonstrated that most of them individually supplemented the industrial Trichoderma reesei CL847 enzymatic cocktail. Unexpectedly, the most striking effect was obtained with the phytopathogen Ustilago maydis that improved the release of total sugars by 57% and of glucose by 22%. Proteomic analyses of the best-performing secretomes indicated a specific enzymatic mechanism of U. maydis that is likely to involve oxido-reductases and hemicellulases. This study provides insight into the lignocellulose-degradation mechanisms by filamentous fungi and allows for the identification of a number of enzymes that are potentially useful to further improve the industrial lignocellulose bioconversion process.
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影响因子：
12.3
作者：
Kubicek CP;Herrera-Estrella A;Seidl-Seiboth V;Martinez DA;Druzhinina IS;Thon M;Zeilinger S;Casas-Flores S;Horwitz BA;Mukherjee PK;Mukherjee M;Kredics L;Alcaraz LD;Aerts A;Antal Z;Atanasova L;Cervantes-Badillo MG;Challacombe J;Chertkov O;McCluskey K;Coulpier F;Deshpande N;von Döhren H;Ebbole DJ;Esquivel-Naranjo EU;Fekete E;Flipphi M;Glaser F;Gómez-Rodríguez EY;Gruber S;Han C;Henrissat B;Hermosa R;Hernández-Oñate M;Karaffa L;Kosti I;Le Crom S;Lindquist E;Lucas S;Lübeck M;Lübeck PS;Margeot A;Metz B;Misra M;Nevalainen H;Omann M;Packer N;Perrone G;Uresti-Rivera EE;Salamov A;Schmoll M;Seiboth B;Shapiro H;Sukno S;Tamayo-Ramos JA;Tisch D;Wiest A;Wilkinson HH;Zhang M;Coutinho PM;Kenerley CM;Monte E;Baker SE;Grigoriev IV
通讯作者：
Grigoriev IV
影响因子：
5
作者：
Battaglia, Evy;Hansen, Sara Fasmer;Leendertse, Anne;Madrid, Susan;Mulder, Harm;Nikolaev, Igor;de Vries, Ronald P.
通讯作者：
de Vries, Ronald P.
影响因子：
3
作者：
Lesage-Meessen, L;Lomascolo, A;Asther, M
通讯作者：
Asther, M
影响因子：
2.4
作者：
Alberto, F.;Navarro, D.;Record, E.
通讯作者：
Record, E.
影响因子：
4.5
作者：
Coleman JJ;Rounsley SD;Rodriguez-Carres M;Kuo A;Wasmann CC;Grimwood J;Schmutz J;Taga M;White GJ;Zhou S;Schwartz DC;Freitag M;Ma LJ;Danchin EG;Henrissat B;Coutinho PM;Nelson DR;Straney D;Napoli CA;Barker BM;Gribskov M;Rep M;Kroken S;Molnár I;Rensing C;Kennell JC;Zamora J;Farman ML;Selker EU;Salamov A;Shapiro H;Pangilinan J;Lindquist E;Lamers C;Grigoriev IV;Geiser DM;Covert SF;Temporini E;Vanetten HD
通讯作者：
Vanetten HD