New Signal Amplification Strategy Using Semicarbazide as Co-reaction Accelerator for Highly Sensitive Electrochemiluminescent Aptasensor Construction.
New Signal Amplification Strategy Using Semicarbazide as Co-reaction Accelerator for Highly Sensitive Electrochemiluminescent Aptasensor Construction.
复制标题
使用氨基脲作为共反应加速剂的新信号放大策略，用于构建高灵敏度电化学发光适体传感器。
DOI：
10.1021/acs.analchem.5b02848
复制
发表时间：
2015-10
影响因子：
7.4
通讯作者：
Chai Yaqin
中科院分区：
文献类型：
--
作者：
Ma Mengnan;Zhuo Ying;Yuan Ruo;Chai Yaqin
一种高灵敏度的电致发光。(ECL)使用氨基脲构建适体传感器。(Sem)作为共反应加速剂，以促进CdTe量子点（CdTe QD）的ECL反应速率和过硫酸盐（S2O8.2-）的共反应物，以增强信号放大。共反应促进剂是一种物质，当它被引入到含有发光体和共反应物的ECL体系中时，它可以与共反应物而不是发光体相互作用，以促进发光体和共反应物的ECL反应速率，从而使ECL信号与仅存在发光体和共反应物的体系相比被显著放大。本工作首先通过Au−N键将Sem和Au纳米粒子（AuNPs）交替组装到空心Au纳米笼（AuNCs）表面，制备了（AuNPs-Sem）n-AuNCs多层纳米材料，用于固定凝血酶（TBA 2）的氨基端检测适体。值得注意的是，具有两个-NH 2末端基团的Sem不仅可以用作组装AuNP和AuNC的交联剂，还可以用作共反应加速剂，以提高CdTe QD和S2O8.2-的ECL反应速率，用于信号放大。在凝血酶（TB）存在的情况下，TBA 2信号探针可以被捕获在基于CdTe QD的传感界面上，以在S2O8.2−溶液中实现显著增强的ECL信号。因此，TBA 2信号探针中的Sem可以加速S2O8.2-的还原，产生更多的SO 4氧化介质。- ，这进一步促进了CdTe量子点的激发态的产生以发光。该传感器对TB浓度在1 × 10−7 ~ 1 nM范围内的定量检测具有很高的灵敏度，检测限为0.03 fM，表明该共反应加速器可以提供一种简单、高效、低成本的信号放大方法，并具有很大的应用潜力.
A highly sensitive electrochemiluminescent.(ECL) aptasensor was constructed using semicarbazide.(Sem) as co-reaction accelerator to promote the ECL reaction.rate of CdTe quantum dots (CdTe QDs) and the co-reactant.of peroxydisulfate (S2O8.2−) for boosting signal amplification..The co-reaction accelerator is a species that when it is.introduced into the ECL system containing luminophore and.co-reactant, it can interact with co-reactant rather than.luminophore to promote the ECL reaction rate of.luminophore and co-reactant; thus the ECL signal is.significantly amplified in comparison with that in which only.luminophore and co-reactant are present. In this work, the.ECL signal probes were first fabricated by alternately assembling the Sem and Au nanoparticles (AuNPs) onto the surfaces of.hollow Au nanocages (AuNCs) via Au−N bond to obtain the multilayered nanomaterials of (AuNPs-Sem)n-AuNCs for.immobilizing amino-terminated detection aptamer of thrombin (TBA2). Notably, the Sem with two -NH2 terminal groups could.not only serve as cross-linking reagent to assemble AuNPs and AuNCs but also act as co-reaction accelerator to enhance the ECL.reaction rate of CdTe QDs and S2O8.2− for signal amplification. With the sandwich-type format, TBA2 signal probes could be.trapped on the CdTe QD-based sensing interface in the presence of thrombin (TB) to achieve a considerably enhanced ECL.signal in S2O8.2− solution. As a result, the Sem in the TBA2 signal probes could accelerate the reduction of S2O8.2− to produce the.more oxidant mediators of SO4.•−, which further boosted the production of excited states of CdTe QDs to emit light. With the.employment of the novel co-reaction accelerator Sem, the proposed ECL biosensor exhibited ultrahigh sensitivity to quantify the.concentration of TB from 1 × 10−7 to 1 nM with a detection limit of 0.03 fM, which demonstrated that the co-reaction.accelerator could provide a simple, efficient, and low-cost approach for signal amplification and hold great potential for other.ECL biosensors construction.
登录
查看更多内容
影响因子：
7.4
作者：
Huang, Ke;Xu, Kailai;Zheng, Chengbin
通讯作者：
Zheng, Chengbin
影响因子：
6.7
作者：
Li, Hailong;Zhai, Junfeng;Sun, Xuping
通讯作者：
Sun, Xuping
影响因子：
9.9
作者：
Au, Leslie;Chen, Yeechi;Zhou, Fei;Camargo, Pedro H. C.;Lim, Byungkwon;Li, Zhi-Yuan;Ginger, David S.;Xia, Younan
通讯作者：
Xia, Younan
影响因子：
7.4
作者：
Yingchang Yu;Chao Lu;Meining Zhang
通讯作者：
Yingchang Yu;Chao Lu;Meining Zhang
具有高双光子效率的竹红菌素金纳米笼用于体外光热/光动力癌症治疗
影响因子：
17.1
作者：
Gao, Liang;Fei, Jinbo;Li, Junbai
通讯作者：
Li, Junbai