The Expression of CXCL10/CXCR3 and Effect of the Axis on the Function of T Lymphocyte Involved in Oral Lichen Planus
The Expression of CXCL10/CXCR3 and Effect of the Axis on the Function of T Lymphocyte Involved in Oral Lichen Planus
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口腔扁平苔藓CXCL10/CXCR3的表达及轴对T淋巴细胞功能的影响
DOI：
10.1007/s10753-018-0934-0
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发表时间：
2018-11
期刊：
影响因子：
5.1
通讯作者：
Fan Y
中科院分区：
文献类型：
--
作者：
Fang JX;Wang C;Shen C;Shan J;Wang XW;Liu L;Fan Y
口腔扁平苔藓（oral lichen planus，OLP）的病因尚不清楚。本研究旨在探讨CXC趋化因子受体3（CXCR 3）及其配体CXC基序趋化因子10（CXCL 10）在OLP发病机制中的作用。我们分别采用实时定量PCR、Western blotting、ELISA和免疫组化方法检测了OLP患者和健康对照组中CXCR 3和CXCL 10的表达。此外，采用Transwell法、CCK-8法和流式细胞术检测了CXCL 10/CXCR 3轴对T淋巴细胞迁移、增殖和凋亡的影响。我们发现CXCR 3和CXCL 10在OLP患者中的表达显著增加。CXCL 10刺激组T淋巴细胞迁移率显著高于对照组和CXCR 3拮抗剂组。拮抗CXCR 3后，T淋巴细胞的迁移能力明显降低，且无论上室培养基中是否加入CXCL 10，迁移细胞数均相似。CXCL 10刺激剂的加入可刺激T淋巴细胞的增殖，但与对照组相比无显著性差异。拮抗CXCR 3后，T淋巴细胞增殖率明显降低。而CXCL 10刺激组、CXCR 3拮抗剂组和对照组的T淋巴细胞凋亡率无显著性差异。由于CXCR 3和CXCL 10表达的改变，以及它们在介导外周血T淋巴细胞定向迁移，影响T淋巴细胞增殖方面的相互作用，提示CXCL 10/CXCR 3轴可能与OLP的免疫机制有关。
The etiology of oral lichen planus (OLP) is still not clear. The purpose of this study was to explore the role of CXC chemokine receptor 3(CXCR3) and its ligand CXC motif chemokine 10(CXCL10) in the pathogenesis of OLP. We examined the expression of CXCR3 and CXCL10 in OLP patients and healthy controls by quantitative real-time PCR, Western blotting, ELISAs, and immunohistochemistry, respectively. Moreover, we detected the effects of CXCL10/CXCR3 axis on T lymphocyte migration, proliferation and apoptosis by Transwell assays, CCK8 assays, and flow cytometry. We found that the expression of CXCR3 and CXCL10 was significantly increased in OLP patients. In addition, T lymphocyte migration rate of CXCL10 stimulation group was significantly higher than that of control and CXCR3 antagonist groups. After antagonizing CXCR3, the migration ability of T lymphocytes was significantly decreased, and regardless of whether CXCL10 was added in the upper chamber culture medium, the number of migrating cells was similar. The addition of CXCL10 stimulant could stimulate the proliferation of T lymphocytes, but there was no significant difference compared with control group. After antagonizing CXCR3, the proliferation rate of T lymphocytes was significantly reduced. However, there were no significant differences in the apoptosis rates of T lymphocytes between CXCL10 stimulation group, antagonist CXCR3 group, and control group. Due to the change of expression in CXCR3 and CXCL10, and its interaction in mediating the directional migration of peripheral blood T lymphocytes, affecting the proliferation of T lymphocytes, it suggests that CXCL10/CXCR3 axis may be related to the immune mechanism of OLP.
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影响因子：
3.8
作者：
Sidahmed AM;León AJ;Bosinger SE;Banner D;Danesh A;Cameron MJ;Kelvin DJ
通讯作者：
Kelvin DJ
DOI：
--
发表时间：
2011-03
期刊：
Iranian journal of immunology : IJI
影响因子：
--
作者：
A. Andalib;Hassan Doulabi;M. Najafi;M. Tazhibi;A. Rezaie
通讯作者：
A. Andalib;Hassan Doulabi;M. Najafi;M. Tazhibi;A. Rezaie
DOI：
10.1900/rds.2009.6.81
发表时间：
2009
期刊：
The review of diabetic studies : RDS
影响因子：
--
作者：
A. Shimada;Y. Oikawa;Yoshifumi Yamada;Y. Okubo;S. Narumi
通讯作者：
A. Shimada;Y. Oikawa;Yoshifumi Yamada;Y. Okubo;S. Narumi
DOI：
--
发表时间：
2010
期刊：
Acta Universitatis Medicinalis Nanjing
影响因子：
--
作者：
H. Nanjing
通讯作者：
H. Nanjing
DOI：
--
发表时间：
2013
期刊：
--
影响因子：
--
作者：
通讯作者：
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