Type II restriction modification system in Ureaplasma parvum OMC-P162 strain.
Type II restriction modification system in Ureaplasma parvum OMC-P162 strain.
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DOI：
10.1371/journal.pone.0205328
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发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Yanagihara I
中科院分区：
文献类型：
--
作者：
Wu HN;Nakura Y;Yoshimura M;Gaddi Tantengco OA;Nomiyama M;Takayanagi T;Fujita T;Yasukawa K;Yanagihara I
从妊娠26周的早产儿胎盘中分离到细小脲原体血清型3株OMC-P162。在这项研究中，我们对OMC-P162的全基因组进行了测序，并将其与从不同临床条件的患者中分离的其他血清3型菌株进行了比较。在OMC-P162中发现了10个独特的基因，其中5个编码假设的蛋白质。其中，UPV_229和UPV_230基因形成了一个操纵子，其开放阅读框被预测分别编码DNA甲基转移酶和一种假设的蛋白质。OMC-P162基因组DNA修饰分析鉴定出n4 -甲基胞嘧啶（m4C）和n6 -甲基腺嘌呤（m6A），但未鉴定出5-甲基胞嘧啶（m5C）。重组蛋白UPV230表现出核酸内切酶活性，并识别CATG序列，导致a和t之间的钝切，该酶活性与培养的OMC-P162相同，表明该酶在OMC-P162中自然表达。我们将这种酶命名为UpaP162。重组蛋白UPV229处理pT7Blue质粒完全阻断UpaP162限制性内切酶活性。这些结果表明UPV_229和UPV_230基因在脲原体OMC-P162中作为II型限制性修饰系统。
Ureaplasma parvum serovar 3 strain, OMC-P162, was isolated from the human placenta of a preterm delivery at 26 weeks’ gestation. In this study, we sequenced the complete genome of OMC-P162 and compared it with other serovar 3 strains isolated from patients with different clinical conditions. Ten unique genes in OMC-P162, five of which encoded for hypothetical proteins, were identified. Of these, genes UPV_229 and UPV_230 formed an operon whose open reading frames were predicted to code for a DNA methyltransferase and a hypothetical protein, respectively. DNA modification analysis of the OMC-P162 genome identified N4-methylcytosine (m4C) and N6-methyladenine (m6A), but not 5-methylocytosine (m5C). UPV230 recombinant protein displayed endonuclease activity and recognized the CATG sequence, resulting in a blunt cut between A and T. This restriction enzyme activity was identical to that of the cultivated OMC-P162 strain, suggesting that this restriction enzyme was naturally expressed in OMC-P162. We designated this enzyme as UpaP162. Treatment of pT7Blue plasmid with recombinant protein UPV229 completely blocked UpaP162 restriction enzyme activity. These results suggest that the UPV_229 and UPV_230 genes act as a type II restriction-modification system in Ureaplasma OMC-P162.
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影响因子：
48
作者：
Flusberg, Benjamin A.;Webster, Dale R.;Lee, Jessica H.;Travers, Kevin J.;Olivares, Eric C.;Clark, Tyson A.;Korlach, Jonas;Turner, Stephen W.
通讯作者：
Turner, Stephen W.
影响因子：
4.2
作者：
Martinez MA;Das K;Saikolappan S;Materon LA;Dhandayuthapani S
通讯作者：
Dhandayuthapani S
影响因子：
3.6
作者：
Gumulak-Smith, J;Teachman, A;Dybvig, K
通讯作者：
Dybvig, K
DOI：
10.1128/genomea.00256-14
发表时间：
2014-05-22
期刊：
Genome announcements
影响因子：
--
作者：
Wu HN;Nakura Y;Motooka D;Nakamura S;Nishiumi F;Ishino S;Kawai Y;Tanaka T;Takeuchi M;Nakayama M;Fujita T;Yanagihara I
通讯作者：
Yanagihara I
影响因子：
14.9
作者：
Roberts RJ;Vincze T;Posfai J;Macelis D
通讯作者：
Macelis D