Immunomodulatory effects of aerobic training in obesity.
Immunomodulatory effects of aerobic training in obesity.
复制标题
DOI：
10.1155/2011/308965
复制
发表时间：
2011
影响因子：
4.6
通讯作者：
Weis M
中科院分区：
文献类型：
--
作者：
Nickel T;Hanssen H;Emslander I;Drexel V;Hertel G;Schmidt-Trucksäss A;Summo C;Sisic Z;Lambert M;Hoster E;Halle M;Weis M
导论.缺乏运动和肥胖是动脉粥样硬化的独立危险因素。我们分析了10周强化运动训练（ET）对肥胖和瘦型运动员的免疫调节能力。在肥胖（ONE：ET≤40 km/周）和瘦运动员（LNE：ET ≤40 km/周和LE：ET≥55 km/周）中研究了先天免疫反应的标志物。方法.通过流式细胞术分析循环树突状细胞（DC）的BDCA-1/-2表达。RT-PCR和Western blot检测TLR-2/-4/-7和MyD 88。通过ELISA分析循环oxLDL水平。结果与其他两组相比，ONE组基线时BDCA-1表达较低（ONE 0.15%; LNE 0.27%; LE 0.33%; P <0.05），但训练后ONE组BDCA-1表达显著增加（+50%; P <0.05）。相比之下，ONE的BDCA-2表达在基线时较高（ONE 0.25%; LNE 0.11%; LE 0.09%; P < .05），而在10周训练期后下降（-27%; P < .05）。与训练前相比，所有三组都发现TLR-4和TLR-7的基因激活以及相应的蛋白质增加（P <0.01/P <0.05）。在ONE中观察到oxLDL水平降低（-61%; P < .05）。结论.强化运动诱导肥胖运动员BDCA-1+ DC和TLR-4/-7增加我们在此描述了新的免疫调节作用，通过定期有氧运动调节肥胖症的先天免疫和促炎细胞因子。
Introduction. Physical inactivity and obesity are independent risk factors for atherosclerosis. We analyzed the immunomodulatory capacity of 10-week intensified exercise training (ET) in obese and lean athletes. Markers of the innate immune response were investigated in obese (ONE: ET≤40 km/week) and lean athletes (LNE: ET≤40 km/week and LE: ET≥55 km/week). Methods. Circulating dendritic cells (DC) were analyzed by flow-cytometry for BDCA-1/-2-expression. TLR-2/-4/-7 and MyD88 were analyzed by RT-PCR and Western blot. Circulating oxLDL levels were analyzed by ELISA. Results. BDCA-1 expression at baseline was lower in ONE compared to both other groups (ONE 0.15%; LNE 0.27%; LE 0.33%; P < .05), but significantly increased in ONE after training (+50%; P < .05). In contrast, BDCA-2 expression at baseline was higher in ONE (ONE 0.25%; LNE 0.11%; LE 0.09%; P < .05) and decreased in ONE after the 10-week training period (−27%; P < .05). Gene activations of TLR-4 and TLR-7 with corresponding protein increase were found for all three groups (P < .01/P < .05) compared to pre training. A reduction of oxLDL levels was seen in ONE (−61%; P < .05). Conclusions. Intensified exercise induces an increase of BDCA-1+ DCs and TLR-4/-7 in obese athletes. We hereby describe new immune modulatory effects, which—through regular aerobic exercise—modulate innate immunity and pro-inflammatory cytokines in obesity.
登录
查看更多内容
影响因子：
32.4
作者：
Hou, Baidong;Reizis, Boris;DeFranco, Anthony L.
通讯作者：
DeFranco, Anthony L.
影响因子：
4.7
作者：
Lamas, O;Marti, A;Martínez, JA
通讯作者：
Martínez, JA
DOI：
10.1054/xc.2001.24203
发表时间：
2001-05-01
期刊：
Clinical excellence for nurse practitioners : the international journal of NPACE
影响因子：
--
作者：
Fletcher, J S;Banasik, J L
通讯作者：
Banasik, J L
影响因子：
4.4
作者：
Craft, N;Bruhn, KW;Miller, JF
通讯作者：
Miller, JF
影响因子：
15.9
作者：
Binder, CJ;Hartvigsen, K;Witztum, JL
通讯作者：
Witztum, JL