Model for adhesion clutch explains biphasic relationship between actin flow and traction at the cell leading edge.
Model for adhesion clutch explains biphasic relationship between actin flow and traction at the cell leading edge.
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DOI：
10.1088/1478-3975/12/3/035002
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发表时间：
2015-05-13
期刊：
影响因子：
2
通讯作者：
Mogilner A
中科院分区：
文献类型：
--
作者：
Craig EM;Stricker J;Gardel M;Mogilner A
细胞运动依赖于细胞前缘动态肌动蛋白-肌球蛋白-粘附网络的不断重组，从而在细胞前缘产生突起，并在细胞与外部环境之间产生牵引力。我们分析了实验测量的肌动蛋白流量、牵引力、肌凝蛋白密度和粘附密度在对照和药物干扰上皮细胞中的空间分布，以建立肌动蛋白-粘附-肌凝蛋白自组织的前沿力学模型。将f -肌动蛋白网络视为粘性凝胶，肌凝蛋白增强黏着离合器接合而肌动蛋白流动减弱黏着离合器接合的模型可以解释测量到的分子分布，并正确预测肌动蛋白流动和牵引应力的空间分布。我们通过比较其预测与测量肌动蛋白流动和牵引应力在细胞快速和缓慢的肌动蛋白聚合速率来测试模型。该模型预测了肌动蛋白黏度和黏附强度对板层-板层边界位置的影响。该模型进一步预测，板足-板层边界的位置对肌凝蛋白收缩水平不是很敏感。
Cell motility relies on the continuous reorganization of a dynamic actin-myosin-adhesion network at the leading edge of the cell, in order to generate protrusion at the leading edge and traction between the cell and its external environment. We analyze experimentally measured spatial distributions of actin flow, traction force, myosin density, and adhesion density in control and pharmacologically perturbed epithelial cells in order to develop a mechanical model of the actin-adhesion-myosin self-organization at the leading edge. A model in which the F-actin network is treated as a viscous gel, and adhesion clutch engagement is strengthened by myosin but weakened by actin flow, can explain the measured molecular distributions and correctly predict the spatial distributions of the actin flow and traction stress. We test the model by comparing its predictions with measurements of the actin flow and traction stress in cells with fast and slow actin polymerization rates. The model predicts how the location of the lamellipodium-lamellum boundary depends on the actin viscosity and adhesion strength. The model further predicts that the location of the lamellipodium-lamellum boundary is not very sensitive to the level of myosin contraction.
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