Atypical flagella assembly and haploid genome coiling during male gamete formation in Plasmodium.
Atypical flagella assembly and haploid genome coiling during male gamete formation in Plasmodium.
复制标题
疟原虫雄性配子形成过程中的非典型鞭毛组装和单倍体基因组卷曲。
DOI：
10.1038/s41467-023-43877-w
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发表时间：
2023-12-13
影响因子：
16.6
通讯作者：
Vaughan, Sue
中科院分区：
文献类型：
--
作者：
Hair, Molly;Moreira-Leite, Flavia;Ferguson, David J. P.;Zeeshan, Mohammad;Tewari, Rita;Vaughan, Sue
疟原虫的配子发生在按蚊体内发生，并且对于有性生殖/分化和向前传播到哺乳动物宿主是必需的。为了更好地了解雄性配子发生的3D组织，我们使用连续块面扫描电子显微镜（SBF-SEM）和连续切片细胞电子断层扫描（ssET）的伯氏疟原虫微配子体检查雄性配子形成过程中的关键结构。我们的数据揭示了一个精心组织的轴丝盘绕在细胞核周围，在相反的方向形成一个中央轴丝带的小配子体。此外，我们还发现小配子的细胞核以一种复杂的结构紧紧地缠绕在轴丝周围，这种结构在小配子出现之前就开始形成。我们的发现详细的3D组织的鞭毛小配子和单倍体基因组突出了一些非典型机制的轴丝组装和单倍体基因组组织在雄配子形成的疟疾寄生虫。配子发生对有性生殖至关重要。Hair等人利用体积电子显微镜观察了伯氏疟原虫雄性小配子轴丝周围的单倍体核的结构组织。
Gametogenesis in Plasmodium spp. occurs within the Anopheles mosquito and is essential for sexual reproduction / differentiation and onwards transmission to mammalian hosts. To better understand the 3D organisation of male gametogenesis, we used serial block face scanning electron microscopy (SBF-SEM) and serial-section cellular electron tomography (ssET) of P. berghei microgametocytes to examine key structures during male gamete formation. Our data reveals an elaborate organisation of axonemes coiling around the nucleus in opposite directions forming a central axonemal band in microgametocytes. Furthermore, we discover the nucleus of microgametes to be tightly coiled around the axoneme in a complex structure whose formation starts before microgamete emergence during exflagellation. Our discoveries of the detailed 3D organisation of the flagellated microgamete and the haploid genome highlight some of the atypical mechanisms of axoneme assembly and haploid genome organisation during male gamete formation in the malaria parasite. Gametogenesis is critical for sexual reproduction. Using volume electron microscopy, Hair et al report the structural organisation of the haploid nucleus coiled around the axoneme of the Plasmodium berghei male microgamete.
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影响因子：
9.8
作者：
Bertiaux E;Balestra AC;Bournonville L;Louvel V;Maco B;Soldati-Favre D;Brochet M;Guichard P;Hamel V
通讯作者：
Hamel V
影响因子：
3
作者：
Kremer, JR;Mastronarde, DN;McIntosh, JR
通讯作者：
McIntosh, JR
DOI：
10.1073/pnas.2110996118
发表时间：
2021-11-09
影响因子：
11.1
作者：
Leung MR;Zenezini Chiozzi R;Roelofs MC;Hevler JF;Ravi RT;Maitan P;Zhang M;Henning H;Bromfield EG;Howes SC;Gadella BM;Heck AJR;Zeev-Ben-Mordehai T
通讯作者：
Zeev-Ben-Mordehai T
影响因子：
7.7
作者：
Höög JL;Lacomble S;O'Toole ET;Hoenger A;McIntosh JR;Gull K
通讯作者：
Gull K
影响因子：
14.9
作者：
Iudin, Andrii;Korir, Paul K.;Somasundharam, Sriram;Weyand, Simone;Cattavitello, Cesare;Fonseca, Neli;Salih, Osman;Kleywegt, Gerard J.;Patwardhan, Ardan
通讯作者：
Patwardhan, Ardan