Presence of CrkI-containing microvesicles in squamous cell carcinomas could have ramifications on tumor biology and cancer therapeutics.
Presence of CrkI-containing microvesicles in squamous cell carcinomas could have ramifications on tumor biology and cancer therapeutics.
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DOI：
10.1038/s41598-022-08905-7
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发表时间：
2022-03-21
影响因子：
4.6
通讯作者：
Shafikhani SH
中科院分区：
文献类型：
--
作者：
Mohamed MF;Al-Khudari S;Cassini-Vieira P;Erra A;Bagabas R;Houser T;Stenson K;Bhayani M;Jelinek MJ;Bishehsari F;Kuzel TM;Shafikhani SH
最近，我们描述了一种现象，即凋亡细胞产生并释放含有 CrkI 的微泡，这些微泡在接触时刺激周围细胞增殖，以补偿自身的死亡。我们将这些微泡称为“ACPSV”，即凋亡代偿性增殖信号微泡。由于免疫细胞和大多数当前的癌症治疗主要通过细胞凋亡来破坏肿瘤细胞，我们进行了一项小型试点研究来评估鳞状细胞癌中也可能产生 ACPSV 的可能性。我们首先评估了原发性和转移性鳞状细胞癌癌细胞系在正常和凋亡条件下产生 ACPSV 的能力。接下来，我们进行了一项试点研究，以评估从 20 名鳞状细胞癌患者身上提取的实体瘤中 ACPSV 的发生情况。两种癌细胞系在凋亡条件下均产生大量 ACPSV。有趣的是，转移性鳞状细胞癌癌细胞系在健康条件下也产生高水平的ACPSV，这表明产生ACPSV的能力可能被这些细胞劫持。重要的是，ACPSV 在所有鳞状细胞癌患者的实体瘤中也含量丰富。癌症中 ACPSV 的检测对肿瘤生物学和癌症治疗具有潜在的重要影响，值得进一步研究。
Recently, we described a phenomenon whereby apoptotic cells generate and release CrkI-containing microvesicles, which stimulate proliferation in surrounding cells upon contact to compensate for their own demise. We termed these microvesicles “ACPSVs” for Apoptotic Compensatory Proliferation Signaling microvesicles. As immune cells and a majority of current cancer therapeutics destroy tumor cells primarily by apoptosis, we conducted a small pilot study to assess the possibility that ACPSVs may also be generated in squamous cell carcinomas. We first evaluated a primary and a metastatic squamous cell carcinoma cancer cell lines for their ability to produce ACPSVs under normal and apoptotic conditions. We next conducted a pilot study to assess the occurrence of ACPSVs in solid tumors extracted from 20 cancer patients with squamous cell carcinomas. Both cancer cell lines produced copious amounts of ACPSVs under apoptotic conditions. Interestingly, the metastatic squamous cell carcinoma cancer cell line also produced high levels of ACPSVs under healthy condition, suggesting that the ability to generate ACPSVs may be hijacked by these cells. Importantly, ACPSVs were also abundant in the solid tumors of all squamous cell carcinoma cancer patients. Detection of ACPSVs in cancer has potentially important ramifications in tumor biology and cancer therapeutics which warrants further investigation.
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