An automated smartphone-based diagnostic assay for point-of-care semen analysis.
An automated smartphone-based diagnostic assay for point-of-care semen analysis.
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DOI：
10.1126/scitranslmed.aai7863
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发表时间：
2017-03-22
影响因子：
17.1
通讯作者：
Shafiee H
中科院分区：
文献类型：
--
作者：
Kanakasabapathy MK;Sadasivam M;Singh A;Preston C;Thirumalaraju P;Venkataraman M;Bormann CL;Draz MS;Petrozza JC;Shafiee H
男性不育症影响了多达12%的世界男性人口，并与各种环境和医疗条件有关。人工显微镜检测和计算机辅助精液分析(CASA)是目前诊断男性不育的标准方法，但这些方法都是劳动密集型、昂贵和以实验室为基础的。对男性不育检测的文化和社会主导的耻辱阻碍了大量男性接受不育检测，特别是在资源有限的非洲国家。我们描述了一种基于智能手机的自动精液分析仪的开发和临床测试，该分析仪旨在定量测量精子密度和活动率，用于照护点男性不育筛查。使用生育诊所的350份临床精液标本，我们的方法可以分析未经洗涤、未经处理的液化精液样本，使用<；5-S平均处理时间，并根据世界卫生组织的指导方针为用户提供精液质量评估，准确率~98%。这项工作表明，微流控技术、光学传感配件以及消费电子产品的进步，特别是智能手机的功能，可以让发达国家和发展中国家的智能手机用户都能进行远程精液质量检测。
Male infertility affects up to 12% of the world’s male population and is linked to various environmental and medical conditions. Manual microscope-based testing and computer-assisted semen analysis (CASA) are the current standard methods to diagnose male infertility; however, these methods are labor-intensive, expensive, and laboratory-based. Cultural and socially dominated stigma against male infertility testing hinders a large number of men from getting tested for infertility, especially in resource-limited African countries. We describe the development and clinical testing of an automated smartphone-based semen analyzer designed for quantitative measurement of sperm concentration and motility for point-of-care male infertility screening. Using a total of 350 clinical semen specimens at a fertility clinic, we have shown that our assay can analyze an unwashed, unprocessed liquefied semen sample with <5-s mean processing time and provide the user a semen quality evaluation based on the World Health Organization (WHO) guidelines with ~98% accuracy. The work suggests that the integration of microfluidics, optical sensing accessories, and advances in consumer electronics, particularly smartphone capabilities, can make remote semen quality testing accessible to people in both developed and developing countries who have access to smartphones.
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影响因子：
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作者：
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通讯作者：
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影响因子：
3.8
作者：
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