Goose Mx and OASL Play Vital Roles in the Antiviral Effects of Type I, II, and III Interferon against Newly Emerging Avian Flavivirus.
Goose Mx and OASL Play Vital Roles in the Antiviral Effects of Type I, II, and III Interferon against Newly Emerging Avian Flavivirus.
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鹅MX和OASL在I型，II和III型干扰素与新兴新兴禽流病毒的抗病毒作用中起着至关重要的作用。
DOI：
10.3389/fimmu.2017.01006
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发表时间：
2017
影响因子：
7.3
通讯作者：
Cheng A
中科院分区：
文献类型：
--
作者：
Chen S;Zhang W;Wu Z;Zhang J;Wang M;Jia R;Zhu D;Liu M;Sun K;Yang Q;Wu Y;Chen X;Cheng A
鸭坦布苏病毒（TMUV）是一种新兴的禽类黄病毒，对禽类具有高致病性，有可能成为人畜共患病原体。本文研究了鹅I、II和III型干扰素（goIFNα、goIFNγ和goIFNλ）对TMUV的分子抗病毒机制，这些干扰素是先天免疫途径的关键成分。我们发现在体内和体外，goIFNs的转录明显受到TMUV感染的驱动，并且在使用goIFNs治疗后，TMUV的滴度和拷贝数明显降低。RNA测序（RNA-seq）结果显示，goIFN刺激触发了一组不同水平的差异表达基因和IFN释放抗感染的正调节反馈回路。两个重要的干扰素刺激基因，goMx和goOASL，被确定为抑制TMUV复制的主力ifn。通过体外瞬时过表达和敲低实验证实了goMx和goOASL的抗病毒作用。总的来说，我们的研究结果确定了鹅Mx和OASL在I型、II型和III型干扰素对TMUV的抗病毒作用中起关键作用。这些结果扩展了我们对鹅ifn介导的信号通路转录谱的理解，并为goifn抗黄病毒感染的抗病毒机制提供了见解。
Duck Tembusu virus (TMUV), an emerging avian flavivirus, is highly pathogenic to birds and has the potential to become a zoonotic pathogen. Here, the molecular antiviral mechanism of goose type I, II, and III interferon (goIFNα, goIFNγ, and goIFNλ), the key components of the innate immune pathway, against TMUV was studied. We found that the transcription of goIFNs was obviously driven by TMUV infection in vivo and in vitro, and the titers and copies of TMUV were significantly reduced following treatment with goIFNs. The results of RNA sequencing (RNA-seq) revealed that goIFN stimulation triggered a set of differentially expressed genes at different levels and a positive regulatory feedback loop of IFN release against infection. Two important interferon-stimulated genes, goMx and goOASL, were identified as workhorse IFNs in the inhibition of TMUV replication. The antiviral effects of goMx and goOASL were confirmed by transient overexpression and knockdown assay in vitro. Overall, our findings defined that goose Mx and OASL play key roles in the antiviral effects of type I, II, and III interferon against the TMUV. These results extend our understanding of the transcriptional profile of the goose IFN-mediated signaling pathway and provide insight into the antiviral mechanism of goIFNs against flavivirus infection.
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影响因子：
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DOI：
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发表时间：
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期刊：
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影响因子：
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