Cherry Valley Ducks Mitochondrial Antiviral-Signaling Protein-Mediated Signaling Pathway and Antiviral Activity Research.
Cherry Valley Ducks Mitochondrial Antiviral-Signaling Protein-Mediated Signaling Pathway and Antiviral Activity Research.
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樱桃谷鸭线粒体抗病毒信号蛋白介导的信号通路和抗病毒活性研究。
DOI：
10.3389/fimmu.2016.00377
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发表时间：
2016
影响因子：
7.3
通讯作者：
Wei L
中科院分区：
文献类型：
--
作者：
Li N;Hong T;Li R;Wang Y;Guo M;Cao Z;Cai Y;Liu S;Chai T;Wei L
线粒体抗病毒信号蛋白（MAVS）是维甲酸诱导基因I（RIG-I）样受体（RLR）介导的信号途径的衔接蛋白，参与先天免疫。在这项研究中，樱桃谷鸭MAVS（duMAVS）从脾脏克隆和分析。duMAVS的N端含有一个caspase激活和募集结构域，C端含有一个脯氨酸富集结构域和一个跨膜结构域。实时定量PCR表明，duMAVS在所有组织中广泛表达。鸭坦布苏病毒（DTMUV）感染后，duMAVS的表达显着上调。在鸭胚成纤维细胞中过表达duMAVS可激活干扰素（IFN）-β、核因子-κB、干扰素调节因子7以及许多下游因子（如Mx、PKR、OAS和IL-8）。RNA干扰进一步证实了duMAVS是IFN-β激活的重要衔接子。抗病毒试验表明，duMAVS可以抑制各种病毒复制（DTMUV，新呼肠孤病毒和鸭瘟病毒）在感染的早期阶段。这些结果表明，duMAVS介导的主要信号通路是其广谱抗病毒能力。本研究将有助于更好地了解鸭的天然免疫系统。
Mitochondrial antiviral-signaling protein (MAVS), an adaptor protein of retinoic acid-inducible gene I (RIG-I)-like receptors (RLRs)-mediated signal pathway, is involved in innate immunity. In this study, Cherry Valley duck MAVS (duMAVS) was cloned from the spleen and analyzed. duMAVS was determined to have a caspase activation and recruitment domain at N-terminal, followed by a proline-rich domain and a transmembrane domain at C-terminal. Quantitative real-time PCR indicated that duMAVS was expressed in all tissues tested across a broad expression spectrum. The expression of duMAVS was significantly upregulated after infection with duck Tembusu virus (DTMUV). Overexpression of duMAVS could drive the activation of interferon (IFN)-β, nuclear factor-κB, interferon regulatory factor 7, and many downstream factors (such as Mx, PKR, OAS, and IL-8) in duck embryo fibroblast cells. What is more, RNA interference further confirmed that duMAVS was an important adaptor for IFN-β activation. The antiviral assay showed that duMAVS could suppress the various viral replications (DTMUV, novel reovirus, and duck plague virus) at early stages of infection. Overall, these results showed that the main signal pathway mediated by duMAVS and it had a broad-spectrum antiviral ability. This research will be helpful to better understanding the innate immune system of ducks.
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