Synergistic activity of polarised osteoblasts inside condensations cause their differentiation.
Synergistic activity of polarised osteoblasts inside condensations cause their differentiation.
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DOI：
10.1038/srep11838
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发表时间：
2015-07-06
影响因子：
4.6
通讯作者：
Ventikos Y
中科院分区：
文献类型：
--
作者：
Kaul H;Hall BK;Newby C;Ventikos Y
成骨前细胞或成软骨前细胞的凝聚是启动骨骼发育的一系列过程的第一步。我们提出了一种有效的、新颖的、基于三维试剂的体外膜内成骨凝聚模型。该模型由系统异质性提供信息，并依赖于相互作用的策略，被证明对捕捉凝结生长的规则敏感，并可用于跟踪单个细胞的活动，以观察其宏观影响。因此，它使以前无法获取的数据变得可用，为探索成骨结构的出现以及正常和异常发育提供了新的见解和新的背景。在凝聚的几个阶段中，我们研究成骨细胞‘埋葬’在它们沉积的类骨体内。成骨细胞分化为骨细胞所需的成骨细胞被包裹的机制仍然是一个猜测的问题，几个假说声称捕捉到了这一重要的转变。对这一转变的计算检验表明，成骨细胞既不关闭也不减缓其基质分泌基因--这是一个广泛持有的观点；它们也不是随机分泌基质。该模型进一步揭示了成骨细胞表现出沉积类骨质的极化行为。这既是对我们对凝结的理解的重要补充，也是对该模型实用性的重要验证。
Condensation of pre-osteogenic, or pre-chondrogenic, cells is the first of a series of processes that initiate skeletal development. We present a validated, novel, three-dimensional agent-based model of in vitro intramembranous osteogenic condensation. The model, informed by system heterogeneity and relying on an interaction-reliant strategy, is shown to be sensitive to ‘rules’ capturing condensation growth and can be employed to track activity of individual cells to observe their macroscopic impact. It, therefore, makes available previously inaccessible data, offering new insights and providing a new context for exploring the emergence, as well as normal and abnormal development, of osteogenic structures. Of the several stages of condensation we investigate osteoblast ‘burial’ within the osteoid they deposit. The mechanisms underlying entrapment – required for osteoblasts to differentiate into osteocytes – remain a matter of conjecture with several hypotheses claiming to capture this important transition. Computational examination of this transition indicates that osteoblasts neither turn off nor slow down their matrix secreting genes – a widely held view; nor do they secrete matrix randomly. The model further reveals that osteoblasts display polarised behaviour to deposit osteoid. This is both an important addition to our understanding of condensation and an important validation of the model’s utility.
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影响因子：
--
作者：
MARVASO, V;BERNARD, GW
通讯作者：
BERNARD, GW
影响因子：
4.2
作者：
BELLOWS, CG;AUBIN, JE;ANTOSZ, ME
通讯作者：
ANTOSZ, ME
影响因子：
4.1
作者：
BHARGAVA, U;BARLEV, M;AUBIN, JE
通讯作者：
AUBIN, JE
影响因子：
4.8
作者：
BELLOWS, CG;AUBIN, JE;HEERSCHE, JNM
通讯作者：
HEERSCHE, JNM
影响因子：
1.5
作者：
EKANAYAKE, S;HALL, BK
通讯作者：
HALL, BK