Storable Cell-Laden Alginate Based Bioinks for 3D Biofabrication.
Storable Cell-Laden Alginate Based Bioinks for 3D Biofabrication.
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DOI：
10.3390/bioengineering10010023
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发表时间：
2022-12-23
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在过去的十年中，三维（3D）生物打印的进展已经取得了相当大的进展。制造包含活细胞的复杂3D结构用于药物发现和组织工程的能力具有巨大的潜力。为了实现成功的临床翻译，需要考虑生物学。从生产地点到临床的储存和运输过程的改进将提高未来临床翻译的成功率。成功3D打印的最重要组成部分之一是“生物墨水”，即用于创建打印结构的载有细胞的生物材料。水凝胶是用于基于挤出的生物打印的优选生物墨水。藻酸盐是一种天然生物聚合物，由于其生物相容性、可调性质、快速凝胶化、低成本和易于修饰以指导细胞行为而被广泛使用。藻酸盐先前已经证明了在受控室温（CRT）储存和运输期间保持细胞活力和功能的能力。这项研究的新奇在于开发了一种简单且具有成本效益的密封系统，由此藻酸盐包封的细胞可以在CRT中储存，然后重新配制成可挤出的生物墨水，用于按需3D生物打印细胞负载结构。据我们所知，使用相同的生物材料（藻酸盐）进行细胞的存储和按需3D生物打印之前尚未进行过研究。使用简单的四步法，其中将含有人脂肪来源的干细胞的交联藻酸盐在脱凝胶化之前储存在CRT中，随后与第二藻酸盐混合。使用基于挤出的生物打印机，发现所得生物墨水的可打印性取决于第二藻酸盐的浓度，其中4%和5%（w/v）是最佳的。在15 °C下储存一周后，藻酸盐包封的人脂肪来源的干细胞表现出88 ± 18%的高活细胞回收率。随后在3D晶格构建体内打印的储存细胞表现出优异的打印后活力和均匀分布。这代表了一种简单的、适应性强的方法，通过这种方法，室温储存和生物织物可以被整合用于按需生物打印。
Over the last decade, progress in three dimensional (3D) bioprinting has advanced considerably. The ability to fabricate complex 3D structures containing live cells for drug discovery and tissue engineering has huge potential. To realise successful clinical translation, biologistics need to be considered. Refinements in the storage and transportation process from sites of manufacture to the clinic will enhance the success of future clinical translation. One of the most important components for successful 3D printing is the ‘bioink’, the cell-laden biomaterial used to create the printed structure. Hydrogels are favoured bioinks used in extrusion-based bioprinting. Alginate, a natural biopolymer, has been widely used due to its biocompatibility, tunable properties, rapid gelation, low cost, and easy modification to direct cell behaviour. Alginate has previously demonstrated the ability to preserve cell viability and function during controlled room temperature (CRT) storage and shipment. The novelty of this research lies in the development of a simple and cost-effective hermetic system whereby alginate-encapsulated cells can be stored at CRT before being reformulated into an extrudable bioink for on-demand 3D bioprinting of cell-laden constructs. To our knowledge the use of the same biomaterial (alginate) for storage and on-demand 3D bio-printing of cells has not been previously investigated. A straightforward four-step process was used where crosslinked alginate containing human adipose-derived stem cells was stored at CRT before degelation and subsequent mixing with a second alginate. The printability of the resulting bioink, using an extrusion-based bioprinter, was found to be dependent upon the concentration of the second alginate, with 4 and 5% (w/v) being optimal. Following storage at 15 °C for one week, alginate-encapsulated human adipose-derived stem cells exhibited a high viable cell recovery of 88 ± 18%. Stored cells subsequently printed within 3D lattice constructs, exhibited excellent post-print viability and even distribution. This represents a simple, adaptable method by which room temperature storage and biofabrication can be integrated for on-demand bioprinting.
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影响因子：
18.9
作者：
Elkhoury K;Morsink M;Sanchez-Gonzalez L;Kahn C;Tamayol A;Arab-Tehrany E
通讯作者：
Arab-Tehrany E
影响因子：
9
作者：
Gao T;Gillispie GJ;Copus JS;Pr AK;Seol YJ;Atala A;Yoo JJ;Lee SJ
通讯作者：
Lee SJ
影响因子：
6.6
作者：
Chung, Johnson H. Y.;Naficy, Sina;Wallace, Gordon G.
通讯作者：
Wallace, Gordon G.
影响因子：
6
作者：
Cooke ME;Rosenzweig DH
通讯作者：
Rosenzweig DH
影响因子：
14
作者：
Klock, G;Pfeffermann, A;Zimmermann, U
通讯作者：
Zimmermann, U