CO2 induced pHi changes in the brain of polar fish: a TauCEST application
CO2 induced pHi changes in the brain of polar fish: a TauCEST application
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CO2 引起极地鱼大脑 pHi 变化：TauCEST 应用
DOI：
10.1002/nbm.3955
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发表时间：
2018
影响因子：
2.9
通讯作者：
Bock C
中科院分区：
文献类型：
--
作者：
Wermter FC;Maus B;Pörtner HO;Dreher W;Bock C
从牛磺酸到水的化学交换饱和转移（CEST）（TauCEST）可用于牛磺酸浓度的体内作图以及在低于37°C的温度下测量细胞内pH（pHi）的相对变化。因此，TauCEST提供了一个机会，调查酸碱调节和生活在低温下的变温动物的神经紊乱，特别是研究海洋酸化（OA）对鱼类神经生理变化的影响。在这里，我们报告了TauCEST成像的首次体内应用。因此，该研究旨在研究TauCEST在广泛的温度（1-37°C）和pH（5.5-8.0）范围内的作用，其动机是在极地鱼类大脑中测量到的高牛磺酸浓度。体外实验结果表明，TauCEST效应在低温范围内表现得尤为明显，而在相应的pH范围内（6.8-7.5），TauCEST效应是严格单调的。为了研究TauCEST成像在1.5°C下对极地鳕鱼（Boreogadus色达）脑的特异性，进行了模拟，表明如果优化实验参数，牛磺酸对体内预期CEST效应的贡献约为65%。saida急性暴露于海水中三种不同浓度的CO2（对照正常碳酸血症;相对中度高碳酸血症OAm= 3300 μatm;高碳酸血症OAh= 4900 μatm）。脑的TauCEST成像显示，与对照测量相比，在约1.5-3%的不同CO2浓度下TauCEST效应显著增加，指示pH或代谢物浓度的变化。1H MR谱的连续记录没有支持体内观察到的TauCEST效应的浓度诱导变化。因此，TauCEST在体内的应用提供了在暴露于CO2的过程中绘制极地鳕鱼脑中pH相对变化的可能性。
Chemical exchange saturation transfer (CEST) from taurine to water (TauCEST) can be used forin vivomapping of taurine concentrations as well as for measurements of relative changes in intracellular pH (pHi) at temperatures below 37°C. Therefore, TauCEST offers the opportunity to investigate acid–base regulation and neurological disturbances of ectothermic animals living at low temperatures, and in particular to study the impact of ocean acidification (OA) on neurophysiological changes of fish. Here, we report the firstin vivoapplication of TauCEST imaging. Thus, the study aimed to investigate the TauCEST effect in a broad range of temperatures (1–37°C) and pH (5.5–8.0), motivated by the high taurine concentration measured in the brains of polar fish. Thein vitrodata show that the TauCEST effect is especially detectable in the low temperature range and strictly monotonic for the relevant pH range (6.8–7.5). To investigate the specificity of TauCEST imaging for the brain of polar cod (Boreogadus saida) at 1.5°C simulations were carried out, indicating a taurine contribution of about 65% to thein vivoexpected CEST effect, if experimental parameters are optimized.B. saidawas acutely exposed to three different CO2concentrations in the sea water (control normocapnia; comparatively moderate hypercapnia OAm= 3300 μatm; high hypercapnia OAh= 4900 μatm). TauCEST imaging of the brain showed a significant increase in the TauCEST effect under the different CO2concentrations of about 1.5–3% in comparison with control measurements, indicative of changes in pHior metabolite concentration. Consecutive recordings of1H MR spectra gave no support for a concentration induced change of thein vivoobserved TauCEST effect. Thus, thein vivoapplication of TauCEST offers the possibility of mapping relative changes in pHiin the brain of polar cod during exposure to CO2.
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DOI：
--
发表时间：
--
期刊：
影响因子：
--
作者：
Naoki Sugita
通讯作者：
Naoki Sugita
影响因子：
2.9
作者：
Justin K. M. Roberts;N. Wade-Jardetzky;O. Jardetsky
通讯作者：
Justin K. M. Roberts;N. Wade-Jardetzky;O. Jardetsky
影响因子：
5.7
作者：
Kogan, Feliks;Singh, Anup;Debrosse, Catherine;Haris, Mohammad;Cai, Kejia;Nanga, Ravi Prakash;Elliott, Mark;Hariharan, Hari;Reddy, Ravinder
通讯作者：
Reddy, Ravinder
影响因子：
2.2
作者：
Pfeuffer, J;Tkác, I;Gruetter, R
通讯作者：
Gruetter, R
CO2对大鼠海马切片细胞内pH变化明显引起的兴奋性传递的影响
影响因子：
2.9
作者：
Junhee Lee;T. Taira;P. Pihlaja;B. Ransom;K. Kaila
通讯作者：
K. Kaila