6-Shogaol attenuates LPS-induced inflammation in BV2 microglia cells by activating PPAR-γ.
6-Shogaol attenuates LPS-induced inflammation in BV2 microglia cells by activating PPAR-γ.
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6-Shogaol 通过激活 PPAR-gamma 减轻 BV2 小胶质细胞中 LPS 诱导的炎症
DOI：
10.18632/oncotarget.16719
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发表时间：
2017-06-27
期刊：
影响因子：
--
通讯作者：
Xie G
中科院分区：
文献类型：
--
作者：
Han Q;Yuan Q;Meng X;Huo J;Bao Y;Xie G
6-Shogaol是一种从植物中分离出来的刺激性物质，已知具有抗肿瘤和抗炎作用。然而，6-Shogaol在LPS激活的BV 2小胶质细胞中的抗炎作用和生物学机制在很大程度上仍然未知。在这项研究中，我们评估了6-Shogaol在LPS激活的BV 2小胶质细胞中的抗炎作用。6-在LPS处理前1 h给予Shogaol。ELISA法检测炎症介质的产生。Western blot法检测NF-κB和PPAR-γ的表达。结果表明，6-Shogaol以浓度依赖性方式抑制LPS诱导的TNF-α、IL-1β、IL-6和PGE 2的产生。此外，6-姜烯酚通过抑制NF-κB p65的磷酸化和核转位来抑制LPS诱导的NF-κB活化。此外，6-姜烯酚还能增加细胞内过氧化物酶体增殖物激活受体-γ的表达。此外，GW 9662对PPAR-γ的抑制作用可对抗6-Shogaol对LPS诱导的炎症介质产生的抑制作用。总之，6-Shogaol通过激活PPAR-γ抑制LPS诱导的炎症。
6-Shogaol, a pungent agent isolated from Zingiber officinale Roscoe, has been known to have anti-tumor and anti-inflammatory effects. However, the anti-inflammatory effects and biological mechanism of 6-Shogaol in LPS-activated BV2 microglia remains largely unknown. In this study, we evaluated the anti-inflammatory effects of 6-Shogaol in LPS-activated BV2 microglia. 6-Shogaol was administrated 1 h before LPS treatment. The production of inflammatory mediators were detected by ELISA. The expression of NF-κB and PPAR-γ were detected by western blot analysis. Our results revealed that 6-Shogaol inhibited LPS-induced TNF-α, IL-1β, IL-6, and PGE2 production in a concentration dependent manner. Furthermore, 6-Shogaol inhibited LPS-induced NF-κB activation by inhibiting phosphorylation and nuclear translocation of NF-κB p65. In addition, 6-Shogaol could increase the expression of PPAR-γ. Moreover, inhibition of PPAR-γ by GW9662 could prevent the inhibition of 6-Shogaol on LPS-induced inflammatory mediator production. In conclusion, 6-Shogaol inhibits LPS-induced inflammation by activating PPAR-γ.
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影响因子：
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