Biosynthesis and Processing of Proteinase 3 in U937 Cells
Biosynthesis and Processing of Proteinase 3 in U937 Cells
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U937 细胞中蛋白酶 3 的生物合成和加工
DOI：
--
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发表时间：
1996
影响因子：
4.8
通讯作者：
J. Hoidal
中科院分区：
文献类型：
--
作者：
N. Rao;G. Rao;B. C. Marshall;J. Hoidal
Proteinase 3 is a human polymorphonuclear leukocyte serine proteinase that degrades elastin in vitro and causes emphysema when administered by intratracheal insufflation into hamsters. Proteinase 3, stored in the azurophilic granules, is expressed in progenitor cells of myeloid origin. In the present study, the biosynthesis, processing, and intracellular transport of the enzyme was investigated in the human myelomonocytic cell line U937. Proteinase 3 is initially identified as a 35-kDa precursor and converted into the 29-kDa mature form within 3 h. By using a combination of techniques including amino-terminal sequencing, we identified the 35-kDa form as a zymogen containing an activation dipeptide but lacking the amino-terminal 25 residues, presumably the result of cleavage by a signal peptidase. Tunicamycin treatment and alkalinization of acidic cell compartments with NHCl did not prevent the processing of the proteinase 3 zymogen into the mature form, suggesting that the enzyme is targeted to the cytoplasmic granules by a mechanism other than the mannose 6-phosphate receptor. Brefeldin A inhibited the zymogen processing, suggesting that the dipeptide cleavage occurred in a post-Golgi organelle. The enzyme responsible for the removal of the dipeptide is a cysteine proteinase since E-64d, a class-specific inhibitor, prevented processing. However, treatment of cells with a dipeptidyl peptidase I inhibitor, Gly-Phe-diazomethyl ketone and with the lysosomotropic agents, NHCl and chloroquine, did not prevent dipeptide cleavage, indicating that the processing enzyme for proteinase 3 is not dipeptidyl peptidase I. In contrast, Gly-Phe-diazomethyl ketone inhibited cleavage of the dipeptide from cathepsin G. This indicates that processing of proteinase 3 is distinct from that of cathepsin G. Proteinase 3 is also processed at the COOH-terminal extension. Cleavage takes place next to Arg-222, suggesting that a trypsin-like proteinase is involved in the COOH-terminal processing.
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DOI：
10.1016/s0021-9258(18)61070-1
发表时间：
1987-07
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
P. Matsudaira
通讯作者：
P. Matsudaira
蛋白酶 3 基因的结构、染色体分配和表达。
DOI：
--
发表时间：
1992
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Sturrock,AB;Franklin,KF;Rao,G;Marshall,BC;Rebentisch,MB;Lemons,RS;Hoidal,JR
通讯作者：
Hoidal,JR
DOI：
10.1016/s0021-9258(18)92854-1
发表时间：
1991-05
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Narayanam V. Rao;Nancy G. Wehner;B. C. Marshall;William R. Gray;Beulah H. Gray;John R. Hoidal
通讯作者：
Narayanam V. Rao;Nancy G. Wehner;B. C. Marshall;William R. Gray;Beulah H. Gray;John R. Hoidal
编码蛋白酶 3（韦格纳肉芽肿自身抗原）的基因定位于人类染色体带 19p13.3。
DOI：
10.1159/000133555
发表时间：
1993
期刊：
Cytogenetics and cell genetics
影响因子：
--
作者：
Sturrock,AB;Espinosa3rd,R;Hoidal,JR;LeBeau,MM
通讯作者：
LeBeau,MM
影响因子：
10.5
作者：
A. Janoff
通讯作者：
A. Janoff