Rice cultivars with differing salt tolerance contain similar cation channels in their root cells.
Rice cultivars with differing salt tolerance contain similar cation channels in their root cells.
复制标题
DOI：
10.1093/jxb/ers052
复制
发表时间：
2012-05
影响因子：
6.9
通讯作者：
Maathuis FJ
中科院分区：
文献类型：
--
作者：
Kavitha PG;Miller AJ;Mathew MK;Maathuis FJ
盐度对全球农业构成重大威胁。水稻是我国主要农作物之一，大多数高产品种对盐敏感。水稻品种间遗传变异的研究表明，根质膜转运蛋白的活性和组成可能是水稻品种特异性耐盐性的基础。在目前的研究中，人们发现，耐盐品种Pokkali保持较高的K+/Na+比相比，盐敏感的IR 20在根以及在芽。利用Na+报告染料，IR 20根原生质体表现出更快的Na+积累比Pokkali原生质体。膜电位测量结果表明，根细胞暴露于Na+在IR 20的去极化大大进一步比Pokkali。这些结果表明，IR 20具有较大的质膜Na+电导。为了评估这是否可能是由于不同的离子通道特性，将Pokkali和IR 20水稻品种的根原生质体膜片钳。电压依赖性K+内向整流器，K+外向整流器，电压无关，非选择性通道与单位电导约35，40，和10 pS，分别进行了鉴定。只有非选择性通道显示出显着的Na+渗透性。有趣的是，在这两个品种中，活性的K+内向整流器大幅下调后，植物生长在盐，但门控，电导，和所有通道类型的活动是非常相似的两个品种。
Salinity poses a major threat for agriculture worldwide. Rice is one of the major crops where most of the high-yielding cultivars are highly sensitive to salinity. Several studies on the genetic variability across rice cultivars suggest that the activity and composition of root plasma membrane transporters could underlie the observed cultivar-specific salinity tolerance in rice. In the current study, it was found that the salt-tolerant cultivar Pokkali maintains a higher K+/Na+ ratio compared with the salt-sensitive IR20 in roots as well as in shoots. Using Na+ reporter dyes, IR20 root protoplasts showed a much faster Na+ accumulation than Pokkali protoplasts. Membrane potential measurements showed that root cells exposed to Na+ in IR20 depolarized considerably further than those of Pokkali. These results suggest that IR20 has a larger plasma membrane Na+ conductance. To assess whether this could be due to different ion channel properties, root protoplasts from both Pokkali and IR20 rice cultivars were patch-clamped. Voltage-dependent K+ inward rectifiers, K+ outward rectifiers, and voltage-independent, non-selective channels with unitary conductances of around 35, 40, and 10 pS, respectively, were identified. Only the non-selective channel showed significant Na+ permeability. Intriguingly, in both cultivars, the activity of the K+ inward rectifier was drastically down-regulated after plant growth in salt but gating, conductance, and activity of all channel types were very similar for the two cultivars.
登录
查看更多内容
影响因子：
6.9
作者：
Amtmann, A;Laurie, S;Sanders, D
通讯作者：
Sanders, D
影响因子：
6.4
作者：
Anil, Veena S.;Krishnamurthy, H.;Mathew, M. K.
通讯作者：
Mathew, M. K.
DOI：
10.1073/pnas.84.12.4108
发表时间：
1987-06-01
影响因子：
11.1
作者：
SCHROEDER, JI;RASCHKE, K;NEHER, E
通讯作者：
NEHER, E
影响因子：
3
作者：
Meier, Silke D.;Kovalchuk, Yury;Rose, Christine R.
通讯作者：
Rose, Christine R.
影响因子：
56.9
作者：
Hirsch, RE;Lewis, BD;Sussman, MR
通讯作者：
Sussman, MR