Ecological niche adaptation of Salmonella Typhimurium U288 is associated with altered pathogenicity and reduced zoonotic potential.
Ecological niche adaptation of Salmonella Typhimurium U288 is associated with altered pathogenicity and reduced zoonotic potential.
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DOI：
10.1038/s42003-021-02013-4
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发表时间：
2021-04-23
影响因子：
5.9
通讯作者：
Kingsley RA
中科院分区：
文献类型：
--
作者：
Kirkwood M;Vohra P;Bawn M;Thilliez G;Pye H;Tanner J;Chintoan-Uta C;Branchu P;Petrovska L;Dallman T;Hall N;Stevens MP;Kingsley RA
新细菌病原体的出现是农业和食品安全的持续挑战。鼠伤寒沙门氏菌是世界范围内食源性疾病的主要原因，猪是主要的人畜共患病宿主。两种系统发育上不同的变体U288和ST 34大约同时出现在英国猪中，但对食品安全带来不同的风险。在这里，我们使用基因组流行病学表明，ST 34占所有S的一半以上。鼠伤寒杆菌感染的人，而U288不到2%。U288进化枝在最近的过去通过获得AMR基因、毒力质粒pU 288 -1中的插入缺失以及编码序列中功能丧失多态性的积累而进化。U288比ST 34分离株复制更慢，对干燥更敏感，在小鼠结肠炎模型和猪中表现出不同的致病性。U288感染在淋巴结中扩散较多，而ST 34在肠内容物中回收较多。这些数据与S.鼠伤寒U288对猪的适应性可能决定其降低的人畜共患病潜力。Mark柯克伍德等人采用流行病学、基因组学、系统发育和实验方法评估肠道沙门氏菌U288和ST 34分离株在影响牲畜和潜在人类方面的进化起源和毒力。他们的结果表明，这些分离株的分子进化与猪的致病性改变和U288的人畜共患病潜力降低有关。
The emergence of new bacterial pathogens is a continuing challenge for agriculture and food safety. Salmonella Typhimurium is a major cause of foodborne illness worldwide, with pigs a major zoonotic reservoir. Two phylogenetically distinct variants, U288 and ST34, emerged in UK pigs around the same time but present different risk to food safety. Here we show using genomic epidemiology that ST34 accounts for over half of all S. Typhimurium infections in people while U288 less than 2%. That the U288 clade evolved in the recent past by acquiring AMR genes, indels in the virulence plasmid pU288-1, and accumulation of loss-of-function polymorphisms in coding sequences. U288 replicates more slowly and is more sensitive to desiccation than ST34 isolates and exhibited distinct pathogenicity in the murine model of colitis and in pigs. U288 infection was more disseminated in the lymph nodes while ST34 were recovered in greater numbers in the intestinal contents. These data are consistent with the evolution of S. Typhimurium U288 adaptation to pigs that may determine their reduced zoonotic potential. Mark Kirkwood et al. take an epidemiological, genomic, phylogenetic, and experimental approach to assess the evolutionary origins and virulence of the U288 and ST34 isolates of Salmonella enterica in affecting livestock and potentially humans. Their results indicate that the molecular evolution of these isolates is associated with altered pathogenicity in pigs and reduced zoonotic potential of U288.
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影响因子：
15.8
作者：
Havelaar AH;Kirk MD;Torgerson PR;Gibb HJ;Hald T;Lake RJ;Praet N;Bellinger DC;de Silva NR;Gargouri N;Speybroeck N;Cawthorne A;Mathers C;Stein C;Angulo FJ;Devleesschauwer B;World Health Organization Foodborne Disease Burden Epidemiology Reference Group
通讯作者：
World Health Organization Foodborne Disease Burden Epidemiology Reference Group
影响因子：
9.4
作者：
Foster, Jeffrey T.;Okinaka, Richard T.;Keim, Paul
通讯作者：
Keim, Paul
影响因子：
3.1
作者：
Barthel, M;Hapfelmeier, S;Hardt, WD
通讯作者：
Hardt, WD
影响因子：
3.3
作者：
通讯作者：
--
DOI：
10.1073/pnas.120163297
发表时间：
2000-06-06
影响因子：
11.1
作者：
Datsenko, KA;Wanner, BL
通讯作者：
Wanner, BL