Characterization of the second and third steps in the enzymatic pathway for microcystin-RR biodegradation by Sphingopyxis sp. USTB-05
Characterization of the second and third steps in the enzymatic pathway for microcystin-RR biodegradation by Sphingopyxis sp. USTB-05
复制标题
Sphingopyxis sp 生物降解微囊藻毒素-RR 酶促途径第二步和第三步的表征。
DOI：
10.1007/s13213-014-0885-0
复制
发表时间：
2014-04
影响因子：
3
通讯作者：
Le Lv
中科院分区：
文献类型：
--
作者：
Chunhua Yin;Hui Wang;QIanqian Xu;Le Lv
微囊藻毒素-RR (MC-RR) 是全球微囊藻毒素 (MC) 的主要变体。我们之前分离出Sphingopyxis sp。 USTB-05，可有效生物降解MC-RR。至少涉及 MC 降解基因编码的三种酶。在第一个基因USTB-05-A成功表达的基础上，进一步从Sphingopyxis sp.中克隆了参与MC-RR生物降解的第二个基因（USTB-05-B，KC513423）和第三个基因（USTB-05-C，KC573527）。 USTB-05 并在大肠杆菌 BL21 (DE3) 中表达。纯化后，这些基因编码的MC降解酶用于催化降解MC-RR。结果表明，USTB-05-B编码的第二种酶可以通过破坏Ala-Arg键将线性MC-RR转化为四肽。 USTB-05-C编码的第三种酶可以裂解线性MC-RR和Adda-Glu-Mdha-Ala四肽的Adda-Glu肽键，产生Adda作为它们的共同产物。这些发现将有助于更好地了解鞘氨醇杆菌 (Sphingopyxis sp.) 对 MC 的生物降解机制。 USTB-05。
Microcystin-RR (MC-RR) is a dominant variant of microcystin (MC) worldwide. We previously isolated Sphingopyxis sp. USTB-05, which can efficiently biodegrade MC-RR. At least three enzymes encoded by MC-degrading genes are involved. Based on the successful expression of the first gene USTB-05-A, the second (USTB-05-B, KC513423) and third (USTB-05-C, KC573527) genes involved in the biodegradation of MC-RR were further cloned from Sphingopyxis sp. USTB-05 and expressed in Escherichia coli BL21 (DE3). After purification, the MC-degrading enzymes encoded by these genes were used for the catalytic degradation of MC-RR. The results demonstrated that the second enzyme encoded by USTB-05-B could convert linear MC-RR to a tetrapeptide by breaking the Ala–Arg bond. The third enzyme encoded by USTB-05-C could cleave Adda-Glu peptide bonds of both linear MC-RR and the tetrapeptide of Adda-Glu-Mdha-Ala, producing Adda as their common product. These findings will help better understand the biodegradation mechanism of MCs by Sphingopyxis sp. USTB-05.
登录
查看更多内容
影响因子：
--
作者：
Haibo Yan;G. Pan;H. Zou;Xian-liang Li;Hao Chen
通讯作者：
Haibo Yan;G. Pan;H. Zou;Xian-liang Li;Hao Chen
鞘氨醇生物降解微囊藻毒素-RR 酶途径第一步的表征。
影响因子：
8.8
作者：
Yan, Hai;Wang, Junfeng;Wang, Hui
通讯作者：
Wang, Hui
DOI：
--
发表时间：
2011
期刊：
China Environmental Science
影响因子：
--
作者：
Li Yu-cheng
通讯作者：
Li Yu-cheng
影响因子：
4.1
作者：
Dziga, Dariusz;Wasylewski, Marcin;Wladyka, Benedykt
通讯作者：
Wladyka, Benedykt
影响因子：
4.5
作者：
Bourne, DG;Riddles, P;Blakeley, RL
通讯作者：
Blakeley, RL