The transcriptome of retinal Müller glial cells.
The transcriptome of retinal Müller glial cells.
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DOI：
10.1002/cne.21730
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发表时间：
2008-07-10
影响因子：
2.5
通讯作者：
Cepko, Constance L.
中科院分区：
文献类型：
--
作者：
Roesch, Karin;Jadhav, Ashutosh P.;Trimarchi, Jeffrey M.;Stadler, Michael B.;Roska, Botond;Sun, Ben B.;Cepko, Constance L.
Müler神经胶质细胞是哺乳动物视网膜中的主要神经胶质细胞。为了确定定义Müler神经胶质细胞特性和功能的分子机制，在Affymetrix微阵列上进行了单细胞基因表达谱分析。对一组高水平表达的基因的鉴定表明，穆勒胶质细胞具有核心转录组，这可能是穆勒胶质细胞的许多功能的基础。细胞周期机制和Notch途径的组成部分以及生长因子、趋化因子和脂蛋白的表达可能允许Müler神经胶质细胞与它们所支持的神经元之间的通信，包括调节神经元的活动。这一方法揭示了一组以前在(Müler)胶质细胞中没有特征的转录本；通过原位杂交验证了其中一些基因的表达。穆勒胶质细胞独有表达的基因被鉴定为新的标记。此外，还获得了在Müler神经胶质细胞中表达Cre的新型BAC转基因小鼠。Müler胶质细胞的分子指纹图谱为进一步研究Müler神经胶质细胞在正常和疾病状态下的发育和功能奠定了基础。
Müller glial cells are the major type of glia in the mammalian retina. To identify the molecular machinery that defines Müller glial cell identity and function, single cell gene expression profiling was performed on Affymetrix microarrays. Identification of a cluster of genes expressed at high levels suggests a Müller glia core transcriptome, which likely underlies many of the functions of Müller glia. Expression of components of the cell cycle machinery and the Notch pathway, as well as of growth factors, chemokines, and lipoproteins might allow communication between Müller glial cells and the neurons that they support, including modulation of neuronal activity. This approach revealed a set of transcripts that were not previously characterized in (Müller) glia; validation of the expression of some of these genes was performed by in situ hybridization. Genes expressed exclusively by Müller glia were identified as novel markers. In addition, a novel BAC transgenic mouse that expresses Cre in Müller glia cells was generated. The molecular fingerprint of Müller glia provides a foundation for further studies of Müller glia development and function in normal and diseased states.
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