Decreased levels of the gelsolin plasma isoform in patients with rheumatoid arthritis.
Decreased levels of the gelsolin plasma isoform in patients with rheumatoid arthritis.
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DOI：
10.1186/ar2520
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发表时间：
2008
影响因子：
4.9
通讯作者：
Bokarewa, Maria
中科院分区：
文献类型：
--
作者：
Osborn, Teresia M.;Verdrengh, Margareta;Stossel, Thomas P.;Tarkowski, Andrej;Bokarewa, Maria
凝溶胶蛋白是一种细胞内肌动蛋白结合蛋白，参与细胞形状变化、细胞运动和细胞凋亡。血浆凝溶胶蛋白是一种细胞外凝溶胶蛋白异构体，在健康个体的血液中循环，浓度为 200 ± 50 mg/L，并且被认为是组织损伤期间细胞外肌动蛋白清除系统的关键组成部分。急性损伤和炎症期间血浆凝溶胶蛋白水平降低，给动物施用重组血浆凝溶胶蛋白可改善脓毒症或烧伤后的结果。在本研究中，我们研究了类风湿关节炎患者的血浆凝溶胶蛋白。使用功能性（芘肌动蛋白成核）测定法测量了 78 名类风湿性关节炎患者的循环和关节内血浆凝溶胶蛋白水平，并与 62 名年龄和性别匹配的健康对照进行了比较。与健康对照相比，类风湿性关节炎患者的循环血浆凝溶胶蛋白水平显着降低（141 ± 32 vs 196 ± 40 mg/L，P = 0.0002）。患者的关节内血浆凝溶胶蛋白水平显着低于配对血浆样本（94 ± 24 vs 141 ± 32 mg/L，P = 0.0001）。除四名患者外，所有患者的滑液中均检测到肌动蛋白，免疫沉淀实验鉴定出凝溶胶蛋白-肌动蛋白复合物。与健康对照相比，类风湿性关节炎患者血浆中凝溶胶蛋白的血浆亚型减少。血浆浓度降低以及滑液中肌动蛋白和凝溶胶蛋白-肌动蛋白复合物的存在表明发炎关节中这种潜在抗炎蛋白的局部消耗。
Gelsolin is an intracellular actin-binding protein involved in cell shape changes, cell motility, and apoptosis. An extracellular gelsolin isoform, plasma gelsolin circulates in the blood of healthy individuals at a concentration of 200 ± 50 mg/L and has been suggested to be a key component of an extracellular actin-scavenging system during tissue damage. Levels of plasma gelsolin decrease during acute injury and inflammation, and administration of recombinant plasma gelsolin to animals improves outcomes following sepsis or burn injuries. In the present study, we investigated plasma gelsolin in patients with rheumatoid arthritis. Circulating and intra-articular levels of plasma gelsolin were measured in 78 patients with rheumatoid arthritis using a functional (pyrene-actin nucleation) assay and compared with 62 age- and gender-matched healthy controls. Circulating plasma gelsolin levels were significantly lower in patients with rheumatoid arthritis compared with healthy controls (141 ± 32 versus 196 ± 40 mg/L, P = 0.0002). The patients' intra-articular plasma gelsolin levels were significantly lower than in the paired plasma samples (94 ± 24 versus 141 ± 32 mg/L, P = 0.0001). Actin was detected in the synovial fluids of all but four of the patients, and immunoprecipitation experiments identified gelsolin-actin complexes. The plasma isoform of gelsolin is decreased in the plasma of patients with rheumatoid arthritis compared with healthy controls. The reduced plasma concentrations in combination with the presence of actin and gelsolin-actin complexes in synovial fluids suggest a local consumption of this potentially anti-inflammatory protein in the inflamed joint.
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影响因子：
3.1
作者：
Dahl, B;Schiodt, FV;Lee, WM
通讯作者：
Lee, WM
DOI：
10.1083/jcb.108.5.1717
发表时间：
1989-05
期刊：
The Journal of cell biology
影响因子：
--
作者：
Kwiatkowski DJ;Janmey PA;Yin HL
通讯作者：
Yin HL
影响因子：
5
作者：
Christofidou-Solomidou, M;Scherpereel, A;DiNubile, MJ
通讯作者：
DiNubile, MJ
影响因子：
--
作者：
CLEMMENSEN, I;HOLUND, B;ANDERSEN, RB
通讯作者：
ANDERSEN, RB
影响因子：
4.1
作者：
DUELAND, S;NENSETER, MS;DREVON, CA
通讯作者：
DREVON, CA