Microbial Distribution in a Hydrothermal Plume of the Southwest Indian Ridge
Microbial Distribution in a Hydrothermal Plume of the Southwest Indian Ridge
复制标题
西南印度洋中脊热液羽流中的微生物分布
DOI：
10.1080/01490451.2015.1048393
复制
发表时间：
2016-01
影响因子：
2.3
通讯作者：
Peng Xiaotong
中科院分区：
文献类型：
--
作者：
Li Jiangtao;Zhou Huaiyang;Fang Jiasong;Wu Zijun;Peng Xiaotong
摘要热液羽流广泛分布于全球扩张脊，但很少有人对它们进行微生物研究。最近被确认为独特的新一类洋中脊系统的超低速扩张海脊为热液系统研究提供了惊喜和新的见解。2010年，从超低速扩张的西南印度洋脊（SWIR）不同深度收集了一套水柱样本，包括热液羽流样本和环境海水。我们使用分子方法，如克隆文库，变性梯度凝胶电泳（DGGE）和定量PCR，以确定热液羽和海水中的微生物群落组成及其空间变异性。系统发育分析表明，柱古菌门中α-变形菌门和γ-变形菌门的成员以及海洋类群I的成员占绝对优势。在热液羽流中，古菌种群在空间上是均匀的，而细菌的组成是异质的，在不同的深度有显着不同。此外，几个谱系，密切相关的已知锰（II）氧化剂被发现是丰富的，甚至占主导地位的羽细菌群落。DGGE谱带分析表明，热液羽流样品与环境海水样品的微生物组成没有显著差异。综上所述，我们推断，微生物群落中的短波红外热液羽来源于周围的海水，而不是从海底喷口衍生的壁龛。本文首次报道了西南印度洋海岭热液柱和环境海水中微生物群落结构的特征。
abstract Hydrothermal plumes are widely distributed throughout the global spreading ridges, yet few of them are microbiologically explored. The ultraslow-spreading ridges, recently recognized as a unique, new class of mid-ocean-ridge system, have provided surprises and new insights in hydrothermal system research. A suite of water column samples including both hydrothermal plume samples and ambient seawater were collected at different depths from the ultraslow-spreading Southwest Indian Ridge (SWIR) in 2010. We use molecular approaches such as clone libraries, denaturing gradient gel electrophoresis (DGGE) and quantitative PCR to determine microbial community compositions and their spatial variability within the hydrothermal plume and seawater. Phylogenetic analysis showed that plume samples were mainly dominated by members of α-Proteobacteria and γ-Proteobacteria and members of marine group I group within the Crenarchaeota. Within the hydrothermal plume, archaeal populations were spatially homogeneous, while bacterial compositions were heterogeneous and remarkably distinct at different depths. Moreover, several lineages, closely related to known Mn(II) oxidizers were found to be abundant and even predominant within the plume bacterial communities. DGGE band patterns showed that there was no significant difference in microbial compositions between the samples of hydrothermal plume and ambient seawater. Taken together, we inferred that microbial communities in the SWIR hydrothermal plumes were sourced from ambient seawater rather than from seafloor vent-derived niches. This is the first report on the characteristics of microbial community structures in hydrothermal plume and ambient seawater in the Southwest Indian Ridge.
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影响因子：
64.8
作者：
C. D. Winn;D. Karl;G. Massoth
通讯作者：
C. D. Winn;D. Karl;G. Massoth
影响因子：
6.4
作者：
Cao H;Wang Y;Lee OO;Zeng X;Shao Z;Qian PY
通讯作者：
Qian PY
DOI：
10.1099/00207713-50-2-835
发表时间：
2000-03
影响因子：
2.8
作者：
A. Maruyama;D. Honda;Hiroyuki Yamamoto;K. Kitamura;T. Higashihara
通讯作者：
A. Maruyama;D. Honda;Hiroyuki Yamamoto;K. Kitamura;T. Higashihara
影响因子：
5
作者：
Dick, Gregory J.;Clement, Brian G.;Tebo, Bradley M.
通讯作者：
Tebo, Bradley M.
影响因子：
5.8
作者：
Huber, T;Faulkner, G;Hugenholtz, P
通讯作者：
Hugenholtz, P