In vivo visualization of characteristics of renal microcirculation in hypertensive and diabetic rats.
In vivo visualization of characteristics of renal microcirculation in hypertensive and diabetic rats.
复制标题
高血压和糖尿病大鼠肾脏微循环特征的体内可视化。
DOI：
10.1152/ajprenal.2001.281.3.f571
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
F. Kajiya
中科院分区：
文献类型：
--
作者：
Tokunori Yamamoto;Y. Tomura;Hiroyoshi Tanaka;F. Kajiya
我们开发了一个视频显微镜系统与电荷耦合器件相机和评价它在正常的肾小球微循环的调查[Wistar-Kyoto（WKY）]，自发性高血压（SHR），和链脲霉素诱导的糖尿病大鼠（STZ）。在WKY中，输入小动脉的直径（Af）为11.9 ± 0.7 μ m，输出小动脉的直径（Ef）为8.9 ± 0.7 μ m。在连续记录血压和肾血流量的同时，可同时显示每个肾小球中的Af和Ef。在SHR，Af直径收缩到WKY的约60%。在所有三个组中，在给予钙通道拮抗剂巴尼地平（1-10 μ g/kg iv）后观察到Af和Ef的剂量依赖性扩张。在WKY中未观察到Af/Ef直径比（Af/Ef比）的变化。在SHR中，Af/Ef比值显著增加，因为巴尼地平给药后Af显著扩张。与此相反，巴尼地平扩大Ef在STZ，造成显着降低Af/Ef的比例。该系统可以同时分析在体肾小球微循环和体循环，从而可以更直接地研究病理动物肾小球微循环的特点和急性变化。
We developed a videomicroscope system with a charge-coupled device camera and evaluated it in the investigation of the glomerular microcirculation in normal [Wistar-Kyoto (WKY)], spontaneously hypertensive (SHR), and streptoyotocin-induced diabetic rats (STZ). In WKY, the diameter of the afferent arterioles (Af) was 11.9 +/- 0.7 microm and that of the efferent arterioles (Ef) was 8.9 +/- 0.7 microm. Af and Ef in each glomerulus could be visualized simultaneously with continuous recording of blood pressure and renal blood flow. In SHR, Af diameter was constricted to approximately 60% of that in WKY. A dose-dependent dilation of Af and Ef was observed after administration of barnidipine (1-10 microg/kg iv), a calcium channel antagonist, in all three groups. No change was seen in the Af-to-Ef diameter ratio (Af/Ef ratio) in WKY. In SHR, the Af/Ef ratio increased significantly because of the marked dilation of Af after barnidipine administration. In contrast, barnidipine dilated Ef in STZ, causing a significant reduction in the Af/Ef ratio. This system can analyze in vivo glomerular microcirculation and systemic macrocirculation simultaneously, allowing more direct investigation of the characteristics of and acute changes in glomerular microcirculation in pathological animals.
研究致密斑介导的肾小球血流动力学的体外方法。
影响因子：
19.6
作者：
Ito,S;Carretero,OA
通讯作者：
Carretero,OA
压力引起的自发性高血压大鼠的传入小动脉收缩。
DOI：
10.1161/01.hyp.19.2_suppl.ii164
发表时间：
1992
期刊：
Hypertension (Dallas, Tex. : 1979)
影响因子：
--
作者：
Ito,S;Juncos,LA;Carretero,OA
通讯作者：
Carretero,OA