An in vitro approach to the study of macula densa-mediated glomerular hemodynamics.
An in vitro approach to the study of macula densa-mediated glomerular hemodynamics.
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研究致密斑介导的肾小球血流动力学的体外方法。
DOI：
10.1038/ki.1990.335
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发表时间：
1990
影响因子：
19.6
通讯作者：
Carretero,OA
中科院分区：
文献类型：
--
作者：
Ito,S;Carretero,OA
肾小管肾小球反馈（TGF）在肾小管和母体肾小球之间起作用，对肾脏自动调节和体液和电解质的稳态很重要[1，2]。由于肾小球体（JGA）在特化的肾小管上皮细胞（致密斑）和脉管系统（传入和传出小动脉）之间显示出密切的解剖学关系，因此长期以来一直被认为是TGF的解剖学部位[3]。然而，试图获得直接的证据来支持这一点受到了阻碍的解剖复杂性的JGA。由于JGA位于肾小管层下方，距离肾脏表面有一段距离，致密斑在体内无法直接进行微穿刺，也不能直接观察血管极。为了规避这些限制的体内方法，我们已经开发了一种体外制备方法，其中传入小动脉和致密斑的显微解剖的兔JGA同时进行微灌注。这使我们能够直接研究JGA而不受全身血液动力学和激素因素的影响;此外，可以连续监测和记录传入小动脉的实时图像。与其他制剂不同，只有少数结构会干扰血管极的直接观察，即使在与肾小球重叠的节段中，也可以准确测量小动脉的管腔直径。使用这种制剂，我们已经观察到，增加致密斑灌注液的NaCl浓度会收缩靠近肾小球的节段中的传入小动脉，并且这种收缩会被速尿（一种已知抑制TGF的袢利尿剂）废除。
Tubuloglomerular feedback (TGF), which operates between the tubule and the parent glomerulus, is important to renal autoregulation and homeostasis of body fluid and electrolytes [1, 2]. Since the juxtaglomerular apparatus (JGA) displays an intimate anatomical relationship between the specialized tubular epithelial cells (macula densa) and the vasculature (afferent and efferent arterioles), it has long been suggested as the anatomical site of TGF [3]. However, attempts to obtain direct evidence to support this have been hindered by the anatomical complexity of the JGA. Since the JGA is located beneath the tubular layer at some distance from the surface of the kidney, the macula densa is not accessible to direct micropuncture in vivo, nor is direct observation of the vascular pole possible.To circumvent these limitations of the in vivo approach, we have developed an in vitro preparation in which both the afferent arteriole and the macula densa of a microdissected rabbit JGA are microperfused simultaneously. This allows us to study the JGA directly without being influenced by systemic hemodynamic and hormonal factors; moreover, real-time images of the afferent arteriole can be continuously monitored and recorded. Unlike other preparations, there are only a few structures to interfere with direct observation of the vascular pole, permitting accurate measurement of the luminal diameter of the arterioles even in segments which are overlapped by the glomerulus. Using this preparation, we have observed that increasing the NaCl concentration of the macula densa perfusate constricts the afferent arteriole in the segment close to the glomerulus, and that this constriction is abolished by furosemide, a loop diuretic known to inhibit TGF.
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少数族裔中的艾滋病和静脉注射毒品使用
DOI：
--
发表时间：
1989
期刊：
影响因子：
--
作者：
J. Peterson;R. Bakeman
通讯作者：
R. Bakeman
对美沙酮计划中有 HTLV-III/LAV 感染风险的无症状个体的神经心理和心理社会因素的前瞻性纵向研究：初步结果。
DOI：
10.3109/00207458709043321
发表时间：
1987
期刊：
The International journal of neuroscience
影响因子：
--
作者：
Charles H. Silberstein;F. McKegney;M. O’Dowd;Peter A. Selwyn;Ellie Schoenbaum;Ernest Drucker;Cheryl Feiner;Charles P. Cox;Gerald Friedland
通讯作者：
Gerald Friedland
与艾滋病患者护理相关的卫生专业人员职业倦怠的决定因素。
DOI：
--
发表时间：
1988
期刊：
AIDS (London)
影响因子：
--
作者：
Michael W. Ross;Val Seeger
通讯作者：
Val Seeger
旧金山的艾滋病和静脉注射毒品。
DOI：
--
发表时间：
1988
期刊：
影响因子：
--
作者：
Mossadeq Ar;Chaisson Re
通讯作者：
Chaisson Re
DOI：
10.1176/ps.39.4.439
发表时间：
1988
期刊：
Hospital & community psychiatry
影响因子：
--
作者：
Batki,SL;Sorensen,JL;Faltz,B;Madover,S
通讯作者：
Madover,S