Natural Killer T Cells Restricted by the Monomorphic MHC Class 1b CD1d1 Molecules Behave Like Inflammatory Cells1
Natural Killer T Cells Restricted by the Monomorphic MHC Class 1b CD1d1 Molecules Behave Like Inflammatory Cells1
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受单形 MHC 1b 类 CD1d1 分子限制的自然杀伤 T 细胞表现得像炎症细胞1
作者：
M. Mempel;C. Ronet;F. Suarez;M. Gilleron;G. Puzo;L. Van Kaer;A. Lehuen;P. Kourilsky;G. Gachelin
小鼠Vα14invT细胞(NKT细胞)受CD1d1MHC 1b分子的限制，是T细胞中具有多效性的一个独特亚群。CD1d1限制性的NKT细胞在S.C.诱导的肉芽肿中浸润。注射分枝杆菌磷脂酰肌醇二甘露糖苷(PIM2)，但不注射其脱酰衍生物。早在注射后6小时就可以检测到NKT细胞。虽然PIM2的分子结构符合CD1d1的呈递要求，但抗体阻断和CD1d1−/−小鼠体内野生型NKT细胞过继转移实验表明，CD1d1的表达不是NKT细胞早期募集到注射部位所必需的。IL-12Rβ−/−和CD40−/−小鼠在PIM2攻击后能够募集NKT细胞，证实了这一结论。此外，注射α-半乳糖神经酰胺，一种在CD1d1背景下被识别的NKT细胞配体，只促进了NKT细胞的少量招募。相比之下，注射β-半乳糖神经酰胺，一种与CD1d1结合但不激活CD1d/Tcr途径的合成糖脂，会导致富含NKT细胞的大肉芽肿的发展。最后，局部注射肿瘤坏死因子-α可模拟糖脂的作用。结论：NKT细胞向炎症部位迁移和聚集的方式与先天免疫系统的其他细胞相同，并且迁移和聚集在炎症部位是独立于CD1d1分子的过程。
Murine Vα14invT cells (NKT cells), restricted by the CD1d1 MHC 1b molecules, are a distinctive subset of T cells endowed with pleiotropic functions. CD1d1-restricted NKT cells infiltrate the granulomas induced by the s.c. injection of mycobacterial phosphatidylinositoldimannoside (PIM2) but not of its deacylated derivative. NKT cells are detectable as early as 6 hours following the injection. Although the molecular structure of PIM2 meets the requirements for presentation by CD1d1, Ab blocking and adoptive transfer experiments of wild-type NKT cells into CD1d1−/− mice show that CD1d1 expression is not required for the early recruitment of NKT cells to the injection site. This conclusion was confirmed by the finding that IL-12Rβ−/− and CD40−/− mice were able to recruit NKT cells after PIM2 challenge. Moreover, the injection of α-galactosylceramide, an NKT cell ligand that is recognized in the context of CD1d1, promoted only a minor recruitment of NKT cells. By contrast, injection of β-galactosylceramide, a synthetic glycolipid that binds to CD1d1 but does not activate the CD1d/TCR pathway, resulted in the development of large granulomas rich in NKT cells. Finally, local injection of TNF-α mimics the effect of glycolipids. It is concluded that NKT cells migrate to and accumulate at inflammatory sites in the same way as other cells of the innate immune system and that migration to and accumulation at inflammatory sites are processes independent of the CD1d1 molecule.
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影响因子：
32.4
作者：
Mendiratta, SK;Martin, WD;VanKaer, L
通讯作者：
VanKaer, L
DOI：
--
发表时间：
1997-07
期刊：
The Journal of Experimental Medicine
影响因子：
--
作者：
M. Exley;Jorge A. Garcia;S. Balk;S. Porcelli
通讯作者：
M. Exley;Jorge A. Garcia;S. Balk;S. Porcelli
DOI：
10.1152/ajpgi.00269.2018
发表时间：
2019-05-01
影响因子：
4.5
作者：
Lee, Kuei-Chuan;Chen, Peng;Schnabl, Bernd
通讯作者：
Schnabl, Bernd
生物素化 α-半乳糖神经酰胺的合成及其对免疫系统和 CD1 分子的影响。
DOI：
10.1021/jm990054n
发表时间：
1999
期刊：
Journal of medicinal chemistry.
影响因子：
--
作者：
Sakai,T;Naidenko,OV;Iijima,H;Kronenberg,M;Koezuka,Y
通讯作者：
Koezuka,Y