Exacerbation of Brain Injury by Post-Stroke Exercise Is Contingent Upon Exercise Initiation Timing.
Exacerbation of Brain Injury by Post-Stroke Exercise Is Contingent Upon Exercise Initiation Timing.
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DOI：
10.3389/fncel.2017.00311
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发表时间：
2017
影响因子：
5.3
通讯作者：
Ding Y
中科院分区：
文献类型：
--
作者：
Li F;Geng X;Khan H;Pendy JT Jr;Peng C;Li X;Rafols JA;Ding Y
越来越多的证据表明，脑卒中后的物理康复可以降低发病率。然而，中风后运动的有效性似乎取决于运动的开始。这项研究评估了早期运动加剧脑损伤、诱导活性氧（ROS）产生并促进能量衰竭的假设。将230只成年雄性Sprague-Dawley大鼠进行大脑中动脉（MCA）闭塞2 h，随机分为8组，包括2个假性损伤对照组、3个非运动组和3个运动组。再灌注后6 h、24 h和3 d开始运动。运动结束后24小时（非运动对照组在相应时间点），检测梗死体积和凋亡细胞死亡。在运动结束0.5 h后，通过检测ROS、ATP和NADH水平来量化早期脑氧化代谢。此外，我们还测量了血管生成生长因子的蛋白表达，以确定脑卒中后血管生成是否在康复中发挥作用。不出所料，缺血性卒中导致脑梗死、凋亡细胞死亡和ROS生成，NADH和ATP生成减少。再灌注后6 h开始运动可增加梗死面积和凋亡细胞死亡（p < 0.05），但在再灌注后24 h、3 d运动可降低梗死面积和凋亡细胞死亡。6 h时脑损伤加重，ROS水平升高（p < 0.05）， NADH和ATP水平降低（p < 0.05）。综上所述，早期运动加重了脑损伤，早期运动引起的能量衰竭与ROS的产生可能是脑损伤加重的基础。这些结果阐明了运动开始时间可能影响中风后康复的方式。
Accumulating evidence has demonstrated that post-stroke physical rehabilitation may reduce morbidity. The effectiveness of post-stroke exercise, however, appears to be contingent upon exercise initiation. This study assessed the hypothesis that very early exercise exacerbates brain injury, induces reactive oxygen species (ROS) generation, and promotes energy failure. A total of 230 adult male Sprague-Dawley rats were subjected to middle cerebral artery (MCA) occlusion for 2 h, and randomized into eight groups, including two sham injury control groups, three non-exercise and three exercise groups. Exercise was initiated after 6 h, 24 h and 3 days of reperfusion. Twenty-four hours after completion of exercise (and at corresponding time points in non-exercise controls), infarct volumes and apoptotic cell death were examined. Early brain oxidative metabolism was quantified by examining ROS, ATP and NADH levels 0.5 h after completion of exercise. Furthermore, protein expressions of angiogenic growth factors were measured in order to determine whether post-stroke angiogenesis played a role in rehabilitation. As expected, ischemic stroke resulted in brain infarction, apoptotic cell death and ROS generation, and diminished NADH and ATP production. Infarct volumes and apoptotic cell death were enhanced (p < 0.05) by exercise that was initiated after 6 h of reperfusion, but decreased by late exercise (24 h, 3 days). This exacerbated brain injury at 6 h was associated with increased ROS levels (p < 0.05), and decreased (p < 0.05) NADH and ATP levels. In conclusion, very early exercise aggravated brain damage, and early exercise-induced energy failure with ROS generation may underlie the exacerbation of brain injury. These results shed light on the manner in which exercise initiation timing may affect post-stroke rehabilitation.
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影响因子：
2.1
作者：
Ding, Yun-Hong;Li, Jie;Ding, Yuchuan
通讯作者：
Ding, Yuchuan
影响因子：
8.3
作者：
Ergul A;Alhusban A;Fagan SC
通讯作者：
Fagan SC
影响因子：
6.7
作者：
Bernhardt, Julie;Dewey, Helen;Donnan, Geoffrey
通讯作者：
Donnan, Geoffrey
影响因子：
2.9
作者：
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通讯作者：
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影响因子：
5.1
作者：
Geng, Xiaokun;Li, Fengwu;Ding, Yuchuan
通讯作者：
Ding, Yuchuan