Dietary sugars modulate bacterial-fungal interactions in saliva and inter-kingdom biofilm formation on apatitic surface.
Dietary sugars modulate bacterial-fungal interactions in saliva and inter-kingdom biofilm formation on apatitic surface.
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DOI：
10.3389/fcimb.2022.993640
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发表时间：
2022
影响因子：
5.7
通讯作者：
中科院分区：
文献类型：
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细菌和真菌可以相互作用，在口腔中形成界间生物膜。变形链球菌和白色念珠菌经常在唾液和与幼儿龋齿（蛀牙）相关的牙齿生物膜中检测到，龋齿是一种由膳食糖引起的常见口腔疾病。然而，不同的糖如何影响这种细菌-真菌相互作用仍不清楚。在这里，我们研究特定的糖是否影响唾液中的界间相互作用以及随后的仿牙表面生物膜的形成。这些微生物在含有常见膳食糖（葡萄糖和果糖、蔗糖、淀粉及其组合）的唾液中培养，并通过荧光成像和定量计算分析进行分析。然后将唾液中的细菌和真菌细胞转移到羟基磷灰石盘（牙齿模拟物）上，以允许微生物结合和生物膜发育。我们发现了多种细菌-真菌聚集体，其大小、结构和空间组织根据糖的类型而变化。蔗糖和淀粉+蔗糖诱导形成大型混合物种聚集体，其特征是细菌簇与真菌细胞共结合，而大多数单细胞是在没有糖或存在葡萄糖和果糖的情况下发现的。值得注意的是，磷灰石表面的定植和进一步生长都依赖于糖介导的聚集，从而形成具有独特空间组织和 3D 结构的生物膜。淀粉+蔗糖和蔗糖介导的聚集体发育成大型且高度产酸的生物膜，其中包含由复杂的细胞外葡聚糖基质包围的细菌和真菌细胞（酵母和菌丝）的复杂网络。相比之下，源自葡萄糖和果糖介导的联合体（或无糖）的生物膜稀疏地分布在表面上，没有结构整合，主要作为酸原性降低的单个物种生长。这些发现揭示了膳食糖对唾液中跨界相互作用的影响，以及它们如何介导具有独特结构组织和与人类蛀牙相关的不同酸原性的生物膜形成。
Bacteria and fungi can interact to form inter-kingdom biofilms in the oral cavity. Streptococcus mutans and Candida albicans are frequently detected in saliva and in dental biofilms associated with early childhood caries (tooth-decay), a prevalent oral disease induced by dietary sugars. However, how different sugars influence this bacterial-fungal interaction remains unclear. Here, we investigate whether specific sugars affect the inter-kingdom interaction in saliva and subsequent biofilm formation on tooth-mimetic surfaces. The microbes were incubated in saliva containing common dietary sugars (glucose and fructose, sucrose, starch, and combinations) and analyzed via fluorescence imaging and quantitative computational analyses. The bacterial and fungal cells in saliva were then transferred to hydroxyapatite discs (tooth mimic) to allow microbial binding and biofilm development. We found diverse bacterial-fungal aggregates which varied in size, structure, and spatial organization depending on the type of sugars. Sucrose and starch+sucrose induced the formation of large mixed-species aggregates characterized by bacterial clusters co-bound with fungal cells, whereas mostly single-cells were found in the absence of sugar or in the presence of glucose and fructose. Notably, both colonization and further growth on the apatitic surface were dependent on sugar-mediated aggregation, leading to biofilms with distinctive spatial organizations and 3D architectures. Starch+sucrose and sucrose-mediated aggregates developed into large and highly acidogenic biofilms with complex network of bacterial and fungal cells (yeast and hyphae) surrounded by an intricate matrix of extracellular glucans. In contrast, biofilms originated from glucose and fructose-mediated consortia (or without sugar) were sparsely distributed on the surface without structural integration, growing predominantly as individual species with reduced acidogenicity. These findings reveal the impact of dietary sugars on inter-kingdom interactions in saliva and how they mediate biofilm formation with distinctive structural organization and varying acidogenicity implicated with human tooth-decay.
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影响因子：
3.1
作者：
Falsetta, Megan L.;Klein, Marlise I.;Koo, Hyun
通讯作者：
Koo, Hyun
影响因子：
4.4
作者：
Gregoire, S.;Xiao, J.;Koo, H.
通讯作者：
Koo, H.
影响因子：
4.6
作者：
Guo L;McLean JS;Lux R;He X;Shi W
通讯作者：
Shi W
白色念珠菌通过跨王室生物膜衍生的代谢产物刺激链球菌突变体的微殖民发育。
影响因子：
4.6
作者：
Kim D;Sengupta A;Niepa TH;Lee BH;Weljie A;Freitas-Blanco VS;Murata RM;Stebe KJ;Lee D;Koo H
通讯作者：
Koo H
影响因子：
5.7
作者：
Kim HE;Liu Y;Dhall A;Bawazir M;Koo H;Hwang G
通讯作者：
Hwang G