Dimensionality and dynamics in the behavior of C. elegans.
Dimensionality and dynamics in the behavior of C. elegans.
复制标题
线虫行为的维度和动态。
DOI：
10.1371/journal.pcbi.1000028
复制
发表时间：
2008-04-25
影响因子：
4.3
通讯作者：
Ryu WS
中科院分区：
文献类型：
--
作者：
Stephens GJ;Johnson-Kerner B;Bialek W;Ryu WS
分析动物行为的一个主要挑战是在复杂的运动动作中发现一些潜在的简单性。在这里，我们发现线虫所采用的形状空间是低维的，仅四个维度就可以解释95%的形状变异。这些维度提供了蠕虫行为的定量描述，我们部分地重建了这个空间中的动力学“运动方程”。这些动态有多个吸引子，我们发现蠕虫访问这些吸引子是对微弱的热刺激做出快速且几乎完全确定的反应。不同模式之间的刺激相关性表明，通过将刺激与形状空间中蠕虫的状态同步，可以产生更可靠的行为。我们证实了这一预测，有效地实时“控制”了蠕虫。在描述参与行为生物学的基因、蛋白质、神经元和电路方面已经做了大量的工作，但用于量化行为的技术已经落后于在这些领域取得的进展。在这里，我们在一个足够丰富的领域来解决这种不平衡，这个领域足够复杂、自然的行为，也足够简单，以至于可以详尽地探索运动：秀丽线虫在琼脂平板上自由爬行的运动。根据对蠕虫曲率的测量，我们发现自然蠕虫姿态的空间是低维的，几乎可以通过它们沿四个主要的“特征蠕虫”的投影来描述。这些特征蠕虫的动态既提供了经典蠕虫运动的定量描述，如正向爬行、反向和欧米茄转弯，也提供了更微妙的行为的证据，如特定姿势下的暂停状态。我们可以部分地构造这个形状空间的运动方程，在这些动力学中，我们找到了一组吸引子，可以用作行为状态的严格定义。我们对线虫的观察揭示了一种精确而完整的运动语言和蠕虫行为的新方面。我们相信，这对其他生物来说是一个很有希望的教训。
A major challenge in analyzing animal behavior is to discover some underlying simplicity in complex motor actions. Here, we show that the space of shapes adopted by the nematode Caenorhabditis elegans is low dimensional, with just four dimensions accounting for 95% of the shape variance. These dimensions provide a quantitative description of worm behavior, and we partially reconstruct “equations of motion” for the dynamics in this space. These dynamics have multiple attractors, and we find that the worm visits these in a rapid and almost completely deterministic response to weak thermal stimuli. Stimulus-dependent correlations among the different modes suggest that one can generate more reliable behaviors by synchronizing stimuli to the state of the worm in shape space. We confirm this prediction, effectively “steering” the worm in real time. A great deal of work has been done in characterizing the genes, proteins, neurons, and circuits that are involved in the biology of behavior, but the techniques used to quantify behavior have lagged behind the advancements made in these areas. Here, we address this imbalance in a domain rich enough to allow complex, natural behavior yet simple enough so that movements can be explored exhaustively: the motions of Caenorhabditis elegans freely crawling on an agar plate. From measurements of the worm's curvature, we show that the space of natural worm postures is low dimensional and can be almost completely described by their projections along four principal “eigenworms.” The dynamics along these eigenworms offer both a quantitative characterization of classical worm movement such as forward crawling, reversals, and Omega-turns, and evidence of more subtle behaviors such as pause states at particular postures. We can partially construct equations of motion for this shape space, and within these dynamics we find a set of attractors that can be used as a rigorous definition of behavioral state. Our observations of C. elegans reveal a precise and complete language of motion and new aspects of worm behavior. We believe this is a lesson with promise for other organisms.
登录
查看更多内容
DOI：
10.1073/pnas.0605321104
发表时间：
2007-03-27
影响因子：
11.1
作者：
Keller, Andreas;Vosshall, Leslie B.
通讯作者：
Vosshall, Leslie B.
影响因子：
2.5
作者：
Faumont, S;Lockery, SR
通讯作者：
Lockery, SR
DOI：
10.1073/pnas.0409009101
发表时间：
2005-03-01
影响因子：
11.1
作者：
Gray, JM;Hill, JJ;Bargmann, CI
通讯作者：
Bargmann, CI
影响因子：
2
作者：
CROLL, NA
通讯作者：
CROLL, NA
影响因子：
2
作者：
Karbowski, Jan;Cronin, Christopher J.;Sternberg, Paul W.
通讯作者：
Sternberg, Paul W.