Precise modulation of embryonic development through optogenetics.

Precise modulation of embryonic development through optogenetics.
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DOI:
10.1002/dvg.23505
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发表时间:
2022-12
期刊:
Genesis (New York, N.Y. : 2000)
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其他
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在过去的十年中,光遗传学取得了巨大的进步,它使用光敏蛋白来控制活细胞和动物中的信号转导。然而,光遗传学工具的数量不断增加,可能会压倒适当的光遗传学策略的选择。在这项工作中,我们总结了这一新兴领域的最新进展,并强调了无视蛋白光遗传学在研究胚胎发育中的应用,重点是在形态发生,细胞极化,细胞命运决定,组织分化,神经元再生,突触可塑性和发育过程中细胞的去除的光诱导方面获得的新见解。
The past decade has witnessed enormous progress in optogenetics, which uses photo-sensitive proteins to control signal transduction in live cells and animals. The ever-increasing amount of optogenetic tools, however, could overwhelm the selection of appropriate optogenetic strategies. In this work, we summarize recent progress in this emerging field and highlight the application of opsin-free optogenetics in studying embryonic development, focusing on new insights gained into optical induction of morphogenesis, cell polariy, cell fate determination, tissue differentiation, neuronal regeneration, synaptic plasticity, and removal of cells during development.
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