Guided morphogenesis through optogenetic activation of Rho signalling during early Drosophila embryogenesis.
Guided morphogenesis through optogenetic activation of Rho signalling during early Drosophila embryogenesis.
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DOI：
10.1038/s41467-018-04754-z
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发表时间：
2018-06-18
影响因子：
16.6
通讯作者：
De Renzis S
中科院分区：
文献类型：
--
作者：
Izquierdo E;Quinkler T;De Renzis S
在生物体发育过程中，细胞经历了复杂的形状变化，其与个体形态发生过程的因果关系尚不清楚。这种过程的模块化性质表明，应该有可能分离出单个模块，确定足以驱动组织重塑的最低要求集，并重建形态发生。在这里，我们使用光遗传学来重建果蝇胚胎组织中的上皮折叠，否则这些组织就不会发生内陷。我们的研究表明，Rho信号在空间和时间上的精确激活足以触发心尖收缩和组织折叠。诱导沟可发生在沿背侧-腹侧或前后胚轴的任何位置，以响应光遗传激活的空间模式和水平。因此，上皮折叠是Rho信号时空组织和强度的直接函数，它本身就足以驱动组织内化，而不受与内源性内陷过程相关的任何预先确定的条件或分化程序的影响。光遗传学不仅为扰乱形态发生提供了可能性，也为引导形态发生提供了可能。在这里，作者使用这种技术重建果蝇胚胎的上皮折叠，并研究Rho激活强度、根尖收缩和组织内陷之间的关系。
During organismal development, cells undergo complex changes in shape whose causal relationship to individual morphogenetic processes remains unclear. The modular nature of such processes suggests that it should be possible to isolate individual modules, determine the minimum set of requirements sufficient to drive tissue remodeling, and re-construct morphogenesis. Here we use optogenetics to reconstitute epithelial folding in embryonic Drosophila tissues that otherwise would not undergo invagination. We show that precise spatial and temporal activation of Rho signaling is sufficient to trigger apical constriction and tissue folding. Induced furrows can occur at any position along the dorsal–ventral or anterior–posterior embryo axis in response to the spatial pattern and level of optogenetic activation. Thus, epithelial folding is a direct function of the spatio-temporal organization and strength of Rho signaling that on its own is sufficient to drive tissue internalization independently of any pre-determined condition or differentiation program associated with endogenous invagination processes. Optogenetics is opening the possibility to not only perturb morphogenesis, but also to guide it. Here, the authors use this technique to reconstruct epithelial folding in Drosophila embryos and study the relationship between strength of Rho activation, apical constrictions, and tissue invagination.
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影响因子：
64.8
作者：
Martin, Adam C.;Kaschube, Matthias;Wieschaus, Eric F.
通讯作者：
Wieschaus, Eric F.
影响因子：
48
作者：
Kennedy, Matthew J.;Hughes, Robert M.;Peteya, Leslie A.;Schwartz, Joel W.;Ehlers, Michael D.;Tucker, Chandra L.
通讯作者：
Tucker, Chandra L.
DOI：
10.1083/jcb.201603077
发表时间：
2016-08-29
期刊：
The Journal of cell biology
影响因子：
--
作者：
Mason FM;Xie S;Vasquez CG;Tworoger M;Martin AC
通讯作者：
Martin AC
影响因子：
56.9
作者：
Behrndt, Martin;Salbreux, Guillaume;Heisenberg, Carl-Philipp
通讯作者：
Heisenberg, Carl-Philipp
影响因子：
7.7
作者：
Chung, Se Yeon;Kim, Sangjoon;Andrew, Deborah J.
通讯作者：
Andrew, Deborah J.