CircRNA expression profiles and circRNA-miRNA-mRNA crosstalk in allergic rhinitis.
CircRNA expression profiles and circRNA-miRNA-mRNA crosstalk in allergic rhinitis.
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变应性鼻炎中circRNA表达谱和circRNA-miRNA-mRNA串扰
DOI：
10.1016/j.waojou.2021.100548
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发表时间：
2021-06
期刊：
影响因子：
--
通讯作者：
Cheng L
中科院分区：
文献类型：
--
作者：
Qiu CY;Cui XY;Lu MP;Yin M;Xu WY;Zhu XJ;Yang Q;Cheng L
环状rna （circRNAs）参与炎症；然而，它们在变应性鼻炎（AR）中的作用尚不清楚。在本研究中，我们分析了circRNA的表达，并确定了circRNA- mirna - mrna网络，circRNA通过该网络调节AR的发病机制。我们利用高通量测序（HTS）分析了鼻黏膜中circRNA、miRNA和mRNA的表达谱，采用fold-change bbb1.5和p值< 0.05确定了AR中显著差异表达（DE）的circRNA、miRNA和mRNA，并利用生物信息学和统计学分析构建了decircrna - demirna - demmrna串音网络。对基因本体和京都基因基因组百科全书进行路径分析，识别网络中丰富的生物术语；而RT-PCR和Sanger测序则用于确认circrna。HTS共鉴定出264个decircrna，其中与对照组相比，AR中有120个上调，144个下调。通过17个mirna、11个circrna、29个mrna和64个相互作用对构建decircrna - demirna - demmrna串扰网络。这些基因参与Wnt信号通路、TNF生物合成、炎症反应、PI3K-Akt信号通路和toll样受体。在11个decircrna中，hsa_circ_0008668和circTRIQK在AR组织中表达上调，而hsa_circ_0029853和circRNA_01002在AR组织中表达下调。Sanger测序证实了这些环状rna的反向剪接连接。我们构建了一个新的AR decircrna - demirna - demmrna网络，为进一步研究其潜在的分子机制提供了基础。
Circular RNAs (circRNAs) are involved in inflammation; however, their role in allergic rhinitis (AR) remains unclear. In this study, we analyzed circRNA expression and identified a circRNA-miRNA-mRNA network through which circRNAs regulate AR pathogenesis. We analyzed circRNA, miRNA, and mRNA expression profiles in the nasal mucosa by high-throughput sequencing (HTS), using a fold-change >1.5 and p-value < 0.05 to pinpoint significantly differentially expressed (DE) circRNAs, miRNAs, and mRNAs in AR. A DEcircRNA-DEmiRNA-DEmRNA crosstalk network was then constructed using bioinformatics and statistical analysis. Gene ontology and Kyoto encyclopedia of genes and genomes pathway analyses were performed to identify the biological terms enriched in the network; whereas RT-PCR and Sanger sequencing were used to confirm the circRNAs. A total of 264 DEcircRNAs were identified by HTS, including 120 upregulated and 144 downregulated in AR compared to controls. A DEcircRNA-DEmiRNA-DEmRNA crosstalk network was constructed with 17 miRNAs, 11 circRNAs, 29 mRNAs, and 64 interaction pairs. These genes were involved in the Wnt signaling pathway, TNF biosynthesis, inflammatory responses, the PI3K-Akt signaling pathway, and Toll-like receptors. Of the 11 DEcircRNAs, hsa_circ_0008668 and circTRIQK were upregulated, whereas hsa_circ_0029853 and circRNA_01002 were downregulated in AR tissues. Sanger sequencing confirmed the back-splicing junctions of these circRNAs. We constructed a novel DEcircRNA-DEmiRNA-DEmRNA network for AR that provides a basis for future studies to investigate its underlying molecular mechanisms.
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DOI：
10.1007/s00018-017-2688-5
发表时间：
2018-03
期刊：
Cellular and molecular life sciences : CMLS
影响因子：
--
作者：
Holdt LM;Kohlmaier A;Teupser D
通讯作者：
Teupser D
与2型糖尿病患者炎症相关的环状RNA的筛选和circANKRD36的验证
影响因子：
5.4
作者：
Fang Y;Wang X;Li W;Han J;Jin J;Su F;Zhang J;Huang W;Xiao F;Pan Q;Zou L
通讯作者：
Zou L
影响因子：
3.1
作者：
Liu, Jiao;Cao, Zhiwei
通讯作者：
Cao, Zhiwei
影响因子：
4.6
作者：
Barrett, Steven P.;Salzman, Julia
通讯作者：
Salzman, Julia
影响因子：
14.2
作者：
Shamji, Mohamed H.;Durham, Stephen R.
通讯作者：
Durham, Stephen R.