Increased Inflammatory Reaction to Intestinal Ischemia-Reperfusion in Neonatal versus Adult Mice
Increased Inflammatory Reaction to Intestinal Ischemia-Reperfusion in Neonatal versus Adult Mice
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与成年小鼠相比，新生小鼠对肠道缺血再灌注的炎症反应增加
DOI：
10.1055/s-0034-1387945
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发表时间：
2015
影响因子：
1.8
通讯作者：
Kuebler JF
中科院分区：
文献类型：
--
作者：
Klemann C;Feng X;Ginzel M;Vieten G;Lacher M;Ure BM;Kuebler JF
新生儿和早产儿受到肠道炎症的严重威胁。本研究通过比较新生鼠和成年鼠肠道对缺血再灌注的炎症反应，验证了新生鼠肠道易发生炎症的假设。方法将C57BL/6J小鼠新生儿（4天，n= 36）和成年小鼠（4周，n= 12）随机分为缺血再灌注组（IR）和对照组（Con）。采用夹紧肠系膜上动脉（30min）再灌注（4h）的方法诱导IR动物肠缺血。实验结束后，从小肠中提取RNA，通过实时定量逆转录-聚合酶链反应检测趋化因子CXCL1/KC和CXCL2/MIP-2的表达。流式细胞术分析分离细胞群中中性粒细胞内流（Live+ Ly-6G +）。结果我们观察到，在成年和新生小鼠中，IR后所有测量的促炎终点都有明显增加。然而，新生小鼠肠道的炎症反应明显更强，CXCL1/KC表达和中性粒细胞积累明显高于成年小鼠（p< 0.05）。结论新生小鼠肠道对缺血损伤有明显的炎症反应。这种增加的易感性可以帮助解释在坏死性小肠结肠炎等疾病中看到的过度炎症。
AimNeonate and preterm patients are threatened by exaggerated inflammation of the gut. This study tests the hypothesis that the neonatal gut is prone to inflammation, by comparing the inflammatory reaction of neonatal and adult murine intestine to ischemia and reperfusion.MethodsNeonatal (4 days,n= 36) and adult (4 weeks,n= 12) C57BL/6J mice were randomly divided between ischemia-reperfusion (IR) and untreated controls (Con). In IR animal's intestinal ischemia was induced by clamping the superior mesenteric artery (30 minutes) followed by reperfusion (4 hours). After the experiment, RNA was extracted from the small intestines and the expression of the chemokines CXCL1/KC and CXCL2/MIP-2 were determined by quantitative real-time reverse transcription-polymerase chain reaction. Flow cytometry was used to analyze neutrophil influx (Live+ Ly-6G + ) in isolated cell populations.ResultsWe observed a strong increase in all measured proinflammatory endpoints after IR in both adult and neonatal mice. However, the inflammatory reaction was significantly stronger in neonatal murine intestines, with a significantly higher increase in CXCL1/KC expression and neutrophil accumulation as compared with adults (p< 0.05).ConclusionThe intestines of neonatal mice reacted with an increased inflammatory response to the ischemic insult. This increased susceptibility could help to explain the exaggerated inflammation seen in diseases such as necrotizing enterocolitis.
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影响因子：
2.8
作者：
Jawa, Randeep S.;Quist, Erin;Mercer, David W.
通讯作者：
Mercer, David W.
新生鼠巨噬细胞在 Toll 样受体刺激后表现出增强的趋化能力
影响因子：
1.8
作者：
Winterberg T;Vieten G;Feldmann L;Hansen G;Hennig C;Ure BM;Kuebler JF
通讯作者：
Kuebler JF
小鼠肝缺血/再灌注引起的肺损伤期间 CXC 趋化因子的肺部表达增强。
DOI：
10.1006/jsre.1998.5490
发表时间：
1999
期刊：
The Journal of surgical research
影响因子：
--
作者：
Yoshidome,H;Lentsch,AB;Cheadle,WG;Miller,FN;Edwards,MJ
通讯作者：
Edwards,MJ
DOI：
10.1152/ajpgi.00016.2012
发表时间：
2012-07-01
影响因子：
4.5
作者：
MohanKumar, Krishnan;Kaza, Niroop;Maheshwari, Akhil
通讯作者：
Maheshwari, Akhil
抗细胞因子诱导的中性粒细胞趋化单克隆抗体预防大鼠小肠缺血再灌注损伤。
DOI：
10.1006/jsre.1998.5367
发表时间：
1998
期刊：
The Journal of surgical research
影响因子：
--
作者：
A. Yagihashi;T. Tsuruma;K. Tarumi;T. Kameshima;T. Yajima;Y. Yanai;N. Watanabe;K. Hirata
通讯作者：
K. Hirata