Programming in situ immunofluorescence intensities through interchangeable reactions of dynamic DNA complexes.
Programming in situ immunofluorescence intensities through interchangeable reactions of dynamic DNA complexes.
复制标题
DOI：
10.1002/cbic.201200525
复制
发表时间：
2012-12-21
期刊：
影响因子：
--
通讯作者：
Diehl MR
中科院分区：
文献类型：
--
作者：
Zimak J;Schweller RM;Duose DY;Hittelman WN;Diehl MR
抗体报告强度的调节对于各种原位荧光成像分析至关重要。虽然这种控制对于可视化稀疏分子目标通常是必要的，但调整标记强度的能力对于高度多重成像策略也至关重要，其中必须调整标记报告水平以优化动态检测范围并最大限度地减少不同信号之间的串扰。现有的化学放大方法通常缺乏这种控制。在这里，我们证明线性和分支 DNA 复合物可以设计为可互换的构建块，可以组装成有组织的荧光报告复合物。我们表明，对这些复合物之间的 DNA 链置换反应进行编程的能力提供了新的机会，可以确定性地调整与单个抗体偶联的染料数量，以便增加和可控地平衡固定细胞内的标记物水平。
The regulation of antibody reporting intensities is critical to various in situ fluorescence imaging analyses. While such control is often necessary to visualize sparse molecular targets, the ability to tune marker intensities is also essential for highly multiplexed imaging strategies where marker reporting levels must be tuned to both optimize dynamic detection ranges and minimize crosstalk between different signals. Existing chemical amplification approaches generally lack such control. Here, we demonstrate that linear and branched DNA complexes can be designed to function as interchangeable building blocks that can be assembled into organized, fluorescence reporting complexes. We show that the ability to program DNA strand displacement reactions between these complexes offer new opportunities to deterministically tune the number of dyes that are coupled to individual antibodies in order to both increase and controllably balance marker levels within fixed cells.
登录
查看更多内容
影响因子：
3.2
作者：
Ness, JM;Akhtar, RS;Roth, KA
通讯作者：
Roth, KA
影响因子：
48
作者：
Raj, Arjun;van den Bogaard, Patrick;Rifkin, Scott A.;van Oudenaarden, Alexander;Tyagi, Sanjay
通讯作者：
Tyagi, Sanjay
DOI：
10.1073/pnas.0407024101
发表时间：
2004-10-26
影响因子：
11.1
作者：
Dirks, RM;Pierce, NA
通讯作者：
Pierce, NA
影响因子：
3.2
作者：
Hopman, AHN;Ramaekers, FCS;Speel, EJM
通讯作者：
Speel, EJM
影响因子：
56.9
作者：
Femino, A;Fay, FS;Singer, RH
通讯作者：
Singer, RH