Single-cell transcriptomics reveal DHX9 in mature B cell as a dynamic network biomarker before lymph node metastasis in CRC.
Single-cell transcriptomics reveal DHX9 in mature B cell as a dynamic network biomarker before lymph node metastasis in CRC.
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单细胞转录组学揭示成熟 B 细胞中的 DHX9 作为 CRC 淋巴结转移前的动态网络生物标志物
DOI：
10.1016/j.omto.2021.06.004
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发表时间：
2021-09-24
期刊：
影响因子：
--
通讯作者：
Ling F
中科院分区：
文献类型：
--
作者：
Liu H;Zhong J;Hu J;Han C;Li R;Yao X;Liu S;Chen P;Liu R;Ling F
越来越多的证据表明，临近肿瘤组织中的成熟B细胞作为一种中间状态，在晚期结直肠癌（CRC）中是至关重要的，这与低生存率有关。开发预测性生物标志物，在结直肠癌淋巴结转移前检测成熟B细胞的临界点，对于防止不可逆转的恶化至关重要。我们使用动态网络生物标志物（DNB）方法分析了不同阶段结直肠癌样本邻近组织中的B细胞。725个crc衍生的B细胞的单细胞分析揭示了成熟B细胞亚型的出现。使用DNB方法，我们确定了II期是淋巴结转移前的关键时期，并且DNB触发的逆转差异基因在Janus激酶(JAK)-信号转换器和转录激活因子（STAT）通路中富集，涉及B细胞免疫能力。DHX9 （DEAH-box helicase 9）是一种特异的癌旁组织DNB关键基因。从I期到III期，DHX9及其参与B细胞相关通路的近网络基因的动态表达水平在网络水平上发生逆转。综上所述，CRC邻近组织成熟B细胞中的DHX9可能是CRC进展中可预测的生物标志物和潜在的免疫靶点。基于单细胞转录组学，我们采用了一种新的方法，即动态网络生物标志物，来量化结直肠癌（CRC）邻近组织中成熟B细胞在调节网络中的免疫功能，从而预测结直肠癌转移前的关键时期，并发现特异性的生物标志物。
Increasing evidence indicates that mature B cells in the adjacent tumor tissue, both as an intermediate state, are vital in advanced colorectal cancer (CRC), which is associated with a low survival rate. Developing predictive biomarkers that detect the tipping point of mature B cells before lymph node metastasis in CRC is critical to prevent irreversible deterioration. We analyzed B cells in the adjacent tissues of CRC samples from different stages using the dynamic network biomarker (DNB) method. Single-cell profiling of 725 CRC-derived B cells revealed the emergence of a mature B cell subtype. Using the DNB method, we identified stage II as a critical period before lymph node metastasis and that reversed difference genes triggered by DNBs were enriched in the Janus kinase (JAK)-signal transducer and activator of transcription (STAT) pathway involving B cell immune capability. DHX9 (DEAH-box helicase 9) was a specific para-cancerous tissue DNB key gene. The dynamic expression levels of DHX9 and its proximate network genes involved in B cell-related pathways were reversed at the network level from stage I to III. In summary, DHX9 in mature B cells of CRC-adjacent tissues may serve as a predictable biomarker and a potential immune target in CRC progression. Based on single-cell transcriptomics, we used a novel method named dynamic network biomarkers to quantify the immune function of mature B cells in colorectal cancer (CRC)-adjacent tissues at regulatory networks and thus to predict the critical period before CRC metastasis as well as to uncover specific biomarkers.
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影响因子：
46.9
作者：
Becht, Etienne;McInnes, Leland;Newell, Evan W.
通讯作者：
Newell, Evan W.
影响因子：
16.6
作者：
Aran D;Camarda R;Odegaard J;Paik H;Oskotsky B;Krings G;Goga A;Sirota M;Butte AJ
通讯作者：
Butte AJ
抗原呈递给 B 细胞。
DOI：
10.3410/b2-87
发表时间：
2010-12-17
期刊：
F1000 biology reports
影响因子：
--
作者：
Harwood NE;Batista FD
通讯作者：
Batista FD
影响因子：
--
作者：
Guo, Fei Fei;Cui, Jiu Wei
通讯作者：
Cui, Jiu Wei
DOI：
10.1093/bioinformatics/btp101
发表时间：
2009-04-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Bindea G;Mlecnik B;Hackl H;Charoentong P;Tosolini M;Kirilovsky A;Fridman WH;Pagès F;Trajanoski Z;Galon J
通讯作者：
Galon J