By-passing in vitro screening--next generation sequencing technologies applied to antibody display and in silico candidate selection.
By-passing in vitro screening--next generation sequencing technologies applied to antibody display and in silico candidate selection.
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绕过体外筛选——下一代测序技术应用于抗体展示和计算机候选物选择。
DOI：
10.1093/nar/gkq789
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发表时间：
2010-11
影响因子：
14.9
通讯作者：
Fischer, N.
中科院分区：
文献类型：
--
作者：
Ravn, U.;Gueneau, F.;Baerlocher, L.;Osteras, M.;Desmurs, M.;Malinge, P.;Magistrelli, G.;Farinelli, L.;Kosco-Vilbois, M. H.;Fischer, N.
近年来，下一代测序（NGS）提供的前所未有的DNA测序能力已经彻底改变了基因组研究。结合Illumina测序平台和设计用于将多样性限制于两个CDR 3的scFv文库，已经产生了>1.9 × 107个序列。这种方法允许对文库的多样性进行深入分析，提供了在选择结合两个靶标期间几乎所有scFv的序列信息以及这些富集过程的全局视图。使用最常见的重链CDR 3序列，设计引物以从第三轮选择中拯救scFv。基于序列频率的鉴定检索了使用经典体外筛选遗漏的最有效的scFv和有价值的候选物。因此，通过将NGS与展示技术相结合，可以绕过或补充费力和耗时的前期筛选，并且可以获得对选择过程的有价值的见解，以改善文库设计和对抗体库的理解。
In recent years, unprecedented DNA sequencing capacity provided by next generation sequencing (NGS) has revolutionized genomic research. Combining the Illumina sequencing platform and a scFv library designed to confine diversity to both CDR3, >1.9 × 107 sequences have been generated. This approach allowed for in depth analysis of the library’s diversity, provided sequence information on virtually all scFv during selection for binding to two targets and a global view of these enrichment processes. Using the most frequent heavy chain CDR3 sequences, primers were designed to rescue scFv from the third selection round. Identification, based on sequence frequency, retrieved the most potent scFv and valuable candidates that were missed using classical in vitro screening. Thus, by combining NGS with display technologies, laborious and time consuming upfront screening can be by-passed or complemented and valuable insights into the selection process can be obtained to improve library design and understanding of antibody repertoires.
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影响因子：
3.7
作者：
Dias-Neto E;Nunes DN;Giordano RJ;Sun J;Botz GH;Yang K;Setubal JC;Pasqualini R;Arap W
通讯作者：
Arap W
DOI：
10.1093/bfgp/1.2.189
发表时间：
2002-07-01
期刊：
Briefings in Functional Genomics & Proteomics
影响因子：
--
作者：
Carmen, Sara;Jermutus, Lutz
通讯作者：
Jermutus, Lutz
影响因子：
46.9
作者：
de Wildt, RMT;Mundy, CR;Tomlinson, IM
通讯作者：
Tomlinson, IM
影响因子：
5.6
作者：
DEKRUIF, J;BOEL, E;LOGTENBERG, T
通讯作者：
LOGTENBERG, T
DOI：
10.1073/pnas.88.6.2432
发表时间：
1991-03-01
影响因子：
11.1
作者：
PERSSON, MAA;CAOTHIEN, RH;BURTON, DR
通讯作者：
BURTON, DR