Construction of engineered corpus cavernosum with primary mesenchymal stem cells in vitro.
Construction of engineered corpus cavernosum with primary mesenchymal stem cells in vitro.
复制标题
原代间充质干细胞工程化海绵体的体外构建
DOI：
10.1038/s41598-017-18129-9
复制
发表时间：
2017-12-22
影响因子：
4.6
通讯作者：
Zhao S
中科院分区：
文献类型：
--
作者：
Xie X;Du X;Li K;Chen Y;Guan Y;Zhao X;Niu G;Luan Y;Zhang D;Sun C;Cheng G;Wang J;Xin Q;Xue A;Wang P;Kong F;Liu X;Wang H;Liu Y;Tian C;Yuan M;Liu S;Zhao S
已使用各种方法来重建阴茎。这项研究的目的是研究在体外兔模型中用原代间充质干细胞（MSC）构建工程生态海绵体的可行性。通过既定的脱细胞方法从成年兔子阴茎组织中获得细胞下体矩阵（ACM）。将MSC分离，纯化，然后在ACM上播种以构建工程生态海绵体。随后将种子ACM在孵化器中培养14天。组织学分析表明，在ACM上接种的MSC已增殖并分布良好。 CD31，VWF，平滑肌肌动蛋白（SMA）和肌球蛋白蛋白以及VWF和肌球蛋白mRNA的检测表明，MSC已分化为内皮细胞和平滑肌细胞。此外，通过透射电子显微镜直接观察到工程生态海绵体的细胞形态。这项研究表明，可以在体外使用原代MSC成功构建工程的海绵体。这项技术代表了在体外开发工程生态海绵体的又一步。
Various methods have been used to reconstruct the penis. The objective of this study was to investigate the feasibility of constructing engineered corpus cavernosum with primary mesenchymal stem cells (MSCs) in a rabbit model in vitro. Acellular corporal matrices (ACMs) were obtained from adult rabbit penile tissues through an established decellularization procedure. MSCs were separated, purified, and then seeded on ACMs to construct engineered corpus cavernosum. The seeded ACMs were subsequently cultured in an incubator for 14 days. Histological analyses showed that MSCs seeded on the ACMs had proliferated and were well distributed. Detection of CD31, vWF, smooth muscle actin (SMA), and myosin protein as well as vWF and myosin mRNA revealed that the MSCs had differentiated into endothelial cells and smooth muscle cells. In addition, cell morphology of the engineered corpus cavernosum was directly observed by transmission electron microscopy. This study demonstrated that engineered corpus cavernosum could be successfully constructed using primary MSCs in vitro. This technology represents another step towards developing engineered corpus cavernosum in vitro.
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DOI：
10.1016/j.bjps.2005.05.016
发表时间：
2006-01-01
影响因子：
2.7
作者：
Felici, N;Felici, A
通讯作者：
Felici, A
影响因子：
56.9
作者：
BURNETT, AL;LOWENSTEIN, CJ;SNYDER, SH
通讯作者：
SNYDER, SH
影响因子：
--
作者：
Patel MN;Atala A
通讯作者：
Atala A
影响因子：
3.6
作者：
Monstrey, Stan;Hoebeke, Piet;De Cuypere, Griet
通讯作者：
De Cuypere, Griet
影响因子：
4.5
作者：
Ji, Chenyang;Min, Fuli;Zhang, Jinming
通讯作者：
Zhang, Jinming