Bifidobacterium castoris strains isolated from wild mice show evidence of frequent host switching and diverse carbohydrate metabolism potential.
Bifidobacterium castoris strains isolated from wild mice show evidence of frequent host switching and diverse carbohydrate metabolism potential.
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从野生小鼠中分离出来的双歧杆菌Castoris菌株显示出频繁开关和多种碳水化合物代谢潜力的证据。
DOI：
10.1038/s43705-022-00102-x
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发表时间：
2022-02-25
期刊：
影响因子：
--
通讯作者：
Hall, Lindsay J.
中科院分区：
文献类型：
--
作者：
Kujawska, Magdalena;Raulo, Aura;Millar, Molly;Warren, Fred;Baltrunaite, Laima;Knowles, Sarah C. L.;Hall, Lindsay J.
肠道微生物群双歧杆菌属的成员是广泛分布的人类和动物共生体，据信对其宿主产生有益的影响。然而，对动物相关物种和品系的深入基因组分析有些缺乏，特别是在野生动物种群中。在这里，为了检查宿主特异性和碳水化合物代谢能力的模式，我们对从两个欧洲国家（英国和立陶宛）野生捕获的小型哺乳动物中分离出的双歧杆菌的整个基因组进行了测序。在野生小鼠（黑线姬鼠、黑线姬鼠和黄线姬鼠）中检测到了蓖麻双歧杆菌、动物双歧杆菌和假长双歧杆菌的成员，但在田鼠或鼩鼱中未检测到。蓖麻双歧杆菌是最常回收的双歧杆菌（占所有分离株的 78%），尽管种群中经常含有多种共循环菌株，但大多数菌株仅在单个种群中检测到。系统发育分析表明，与小鼠相关的海狸鼠进化枝并不特定于特定位置或宿主物种，它们在宿主系统发育中的分布与常规宿主转移一致，而不是与宿主-微生物共同多样化一致。包括体外生长测定在内的功能分析表明，小鼠来源的海狸鼠菌株编码了大量碳水化合物活性酶，包括假定的新型糖基水解酶，如壳聚糖酶，以及编码假定的胞外多糖的基因，其中一些可能是通过水平基因转移获得的。总的来说，这些结果为野生动物重要肠道共生体的宿主特异性和基因组能力提供了罕见的基因组水平分析，并揭示了双歧杆菌在进化时间尺度上与其宿主具有不稳定的关系。
Members of the gut microbiota genus Bifidobacterium are widely distributed human and animal symbionts believed to exert beneficial effects on their hosts. However, in-depth genomic analyses of animal-associated species and strains are somewhat lacking, particularly in wild animal populations. Here, to examine patterns of host specificity and carbohydrate metabolism capacity, we sequenced whole genomes of Bifidobacterium isolated from wild-caught small mammals from two European countries (UK and Lithuania). Members of Bifidobacterium castoris, Bifidobacterium animalis and Bifodobacterium pseudolongum were detected in wild mice (Apodemus sylvaticus, Apodemus agrarius and Apodemus flavicollis), but not voles or shrews. B. castoris constituted the most commonly recovered Bifidobacterium (78% of all isolates), with the majority of strains only detected in a single population, although populations frequently harboured multiple co-circulating strains. Phylogenetic analysis revealed that the mouse-associated B. castoris clades were not specific to a particular location or host species, and their distribution across the host phylogeny was consistent with regular host shifts rather than host-microbe codiversification. Functional analysis, including in vitro growth assays, suggested that mouse-derived B. castoris strains encoded an extensive arsenal of carbohydrate-active enzymes, including putative novel glycosyl hydrolases such as chitosanases, along with genes encoding putative exopolysaccharides, some of which may have been acquired via horizontal gene transfer. Overall, these results provide a rare genome-level analysis of host specificity and genomic capacity among important gut symbionts of wild animals, and reveal that Bifidobacterium has a labile relationship with its host over evolutionary time scales.
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影响因子：
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作者：
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影响因子：
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作者：
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DOI：
10.1093/bioinformatics/btp348
发表时间：
2009-08-01
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影响因子：
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