Regulation of leaf maturation by chromatin-mediated modulation of cytokinin responses.
Regulation of leaf maturation by chromatin-mediated modulation of cytokinin responses.
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DOI：
10.1016/j.devcel.2013.01.019
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发表时间：
2013-02-25
影响因子：
11.8
通讯作者：
Wagner, Doris
中科院分区：
文献类型：
--
作者：
Efroni, Idan;Han, Soon-Ki;Kim, Hye Jin;Wu, Miin-Feng;Steiner, Evyatar;Birnbaum, Kenneth D.;Hong, Jong Chan;Eshed, Yuval;Wagner, Doris
植物的芽表现出无限的生长，而它们的进化后代，叶，是有限的。确定的叶生长是由CIN-TCP转录因子调节的，CIN-TCP转录因子促进叶成熟，并且在叶原基中由miR 319负调控。在这里，我们表明，CIN-TCPs降低叶细胞分裂素（CK）的敏感性，植物激素参与抑制分化的拍摄。我们确定SWI/SNF染色质重塑ATP酶BRAHMA（BRM）作为CIN-TCP活动和CK反应的遗传介质。相互作用组筛选进一步揭示了包括BRM在内的SWI/SNF复合物组分优先与bHLH转录因子和bHLH相关的CIN-TCP相互作用。事实上，TCP 4和BRM在植物中相互作用。TCP 4和BRM都与CK反应抑制剂ARR 16的启动子结合，并诱导其表达。在具有降低的CIN-TCP活性的叶中重建ARR 16水平恢复了正常生长。因此，CIN-TCP和BRM一起促进确定的叶生长的阶段特异性修改CK的反应。
Plant shoots display indeterminate growth, while their evolutionary decedents, the leaves, are determinate. Determinate leaf growth is conditioned by the CIN-TCP transcription factors, which promote leaf maturation and which are negatively regulated by miR319 in leaf primordia. Here we show that CIN-TCPs reduce leaf sensitivity to cytokinin (CK), a phytohormone implicated in inhibition of differentiation in the shoot. We identify the SWI/SNF chromatin remodeling ATPase BRAHMA (BRM) as a genetic mediator of CIN-TCP activities and CK responses. An interactome screen further revealed that SWI/SNF complex components including BRM preferentially interacted with bHLH transcription factors and the bHLH-related CIN-TCPs. Indeed, TCP4 and BRM interacted in planta. Both TCP4 and BRM bound the promoter of an inhibitor of CK responses, ARR16, and induced its expression. Reconstituting ARR16 levels in leaves with reduced CIN-TCP activity restored normal growth. Thus, CIN-TCP and BRM together promote determinate leaf growth by stage-specific modification of CK responses.
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影响因子：
56.9
作者：
GAN, SS;AMASINO, RM
通讯作者：
AMASINO, RM
影响因子：
30.8
作者：
Ori, Naomi;Cohen, Aya Refael;Eshed, Yuval
通讯作者：
Eshed, Yuval
影响因子：
11.6
作者：
Greenboim-Wainberg, Y;Maymon, I;Weiss, D
通讯作者：
Weiss, D
影响因子：
11.6
作者：
Han, Soon-Ki;Sang, Yi;Wagner, Doris
通讯作者：
Wagner, Doris
影响因子：
4.6
作者：
Kwon, Chang Seob;Hibara, Ken-Ichiro;Wagner, Doris
通讯作者：
Wagner, Doris