Katanin contributes to interspecies spindle length scaling in Xenopus.
Katanin contributes to interspecies spindle length scaling in Xenopus.
复制标题
katanin有助于爪蟾中的种间主轴长度缩放。
DOI：
10.1016/j.cell.2011.11.014
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发表时间：
2011-12-09
期刊：
影响因子：
64.5
通讯作者：
Heald R
中科院分区：
文献类型：
--
作者：
Loughlin R;Wilbur JD;McNally FJ;Nédélec FJ;Heald R
Bipolar spindles must separate chromosomes by the appropriate distance during cell division, but mechanisms determining spindle length are poorly understood. Based on a 2D model of meiotic spindle assembly, we predicted that higher localized microtubule (MT) depolymerization rates could generate the shorter spindles observed in egg extracts of X. tropicalis compared to X. laevis. We found that katanin-dependent MT severing was increased in X. tropicalis, which lacks an inhibitory phosphorylation site in the p60 catalytic subunit that is present in X. laevis p60. Katanin inhibition lengthened spindles in both species, and kinetochore fibers extended through X. tropicalis spindle poles disrupting them. In both X. tropicalis extracts and the spindle simulation, a threshold number of stable kinetochore fibers could overwhelm MT depolymerization, leading to similar phenotypes. Thus, mechanisms have evolved in different species to scale spindle size and coordinate regulation of multiple MT populations in order to generate a robust steady state structure.
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影响因子：
9.2
作者：
Goshima, G;Wollman, R;Vale, RD
通讯作者：
Vale, RD
DOI：
10.1083/jcb.201006076
发表时间：
2010-12-27
期刊：
The Journal of cell biology
影响因子：
--
作者：
Loughlin R;Heald R;Nédélec F
通讯作者：
Nédélec F
影响因子：
64.8
作者：
Heald, R;Tournebize, R;Karsenti, E
通讯作者：
Karsenti, E
影响因子：
56.9
作者：
Hartman, JJ;Vale, RD
通讯作者：
Vale, RD
影响因子：
7.8
作者：
Brown, Katherine S.;Blower, Michael D.;Heald, Rebecca
通讯作者：
Heald, Rebecca