Bromodeoxyuridine uptake and proliferating cell nuclear antigen expression throughout the colorectal tumor sequence.
Bromodeoxyuridine uptake and proliferating cell nuclear antigen expression throughout the colorectal tumor sequence.
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整个结直肠肿瘤序列中溴脱氧尿苷的摄取和增殖细胞核抗原的表达。
DOI：
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
F. Rossini
中科院分区：
文献类型：
--
作者：
M. Risio;G. Candelaresi;F. Rossini
在体外溴脱氧尿苷的摄取和增殖细胞核抗原（PCNA）的表达进行了评价，在直肠粘膜的对照组和大肠腺瘤和腺癌的结直肠肿瘤。两种标记指数随着肿瘤进展沿着路径逐渐增加，它们之间的差异也是如此（PCNA标记指数总是大于溴脱氧尿苷）。它们之间的相关性是相当接近的控制和腺瘤与低度异型增生，而没有显着的线性关系，在腺瘤与高度异型增生或腺癌。因此，PCNA的进行性增加似乎与PCNA合成的过度增殖和肿瘤性失调有关。在结直肠肿瘤患者的粘膜中，PCNA标记显示过度增殖，但溴脱氧尿苷检测到的增殖区室没有向表面移动。因此，PCNA表达不是肠粘膜中肿瘤转化风险的足够敏感的标志物。
In vitro uptake of bromodeoxyuridine and expression of proliferating cell nuclear antigen (PCNA) were evaluated histochemically in rectal mucosa of control subjects and subjects with colorectal neoplasia in large intestine adenomas and adenocarcinomas. Both labeling indices progressively increased along the path of tumor progression, as did the difference between them (PCNA labeling indices were always greater than those of bromodeoxyuridine). The correlation between them was fairly close in the controls and in adenomas with low-grade dysplasia, whereas no significant linear relations were noted in adenomas with high-grade dysplasia or in adenocarcinomas. The progressive increase in PCNA would thus seem to be related to both hyperproliferation and neoplastic deregulation of PCNA synthesis. In the mucosa of subjects with colorectal neoplasia, PCNA labeling revealed hyperproliferation but not the surface-wards shift of the proliferative compartment detected by bromodeoxyuridine. PCNA expression, therefore, is not a sufficiently sensitive marker of the risk of tumor transformation in the intestinal mucosa.
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一种快速、准确的免疫组织化学方法，用于标记消化道中的增殖细胞。
影响因子：
29.4
作者：
Lacy,ER;Kuwayama,H;Cowart,KS;King,JS;Deutz,AH;Sistrunk,S
通讯作者：
Sistrunk,S
影响因子：
3.7
作者：
KURKI, P;VANDERLAAN, M;TAN, EM
通讯作者：
TAN, EM
DOI：
10.1016/s0021-9258(18)80079-5
发表时间：
1989-08
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
G. Morris;M. Mathews
通讯作者：
G. Morris;M. Mathews
胃肠癌易感性增加的生物标志物：在人类受试者癌症预防研究中的新应用。
影响因子：
11.2
作者：
Lipkin,M
通讯作者：
Lipkin,M