Biased distribution of recombination sites within S regions upon immunoglobulin class switch recombination induced by transforming growth factor beta and lipopolysaccharide.
Biased distribution of recombination sites within S regions upon immunoglobulin class switch recombination induced by transforming growth factor beta and lipopolysaccharide.
复制标题
DOI：
10.1084/jem.175.6.1539
复制
发表时间：
1992-06-01
影响因子：
15.3
通讯作者：
YAMAGISHI, H
中科院分区：
文献类型：
--
作者：
IWASATO, T;ARAKAWA, H;SHIMIZU, A;HONJO, T;YAMAGISHI, H
我们已经表征了来自成年小鼠脾细胞的染色体外环状DNA，所述脾细胞被细菌脂多糖（LPS）和转化生长因子β（TGF-β）诱导转换为免疫球蛋白A（伊加），并且鉴定了S mu/S γ 3、S mu/S γ 2、S mu/S α、S γ 3/S α和S γ 2/S α重组体的断裂点。S mu重组供体位点聚集在S mu区域的3'半部分，而S alpha重组受体位点聚集在S alpha区域的5'半部分。此外，S γ区的供体和受体位点也分别聚集在3'和5'部分。这些网站的偏好是在鲜明的对比，分散分布的S μ/S γ 1断点内的S μ和S γ 1区域后，由LPS和白细胞介素4诱导的IgG 1开关。我们的研究结果支持的假设，TGF-β增加开关重组事件的频率伊加和开关重组伊加往往通过连续重组的S亩/S γ和S γ/S α。
We have characterized extrachromosomal circular DNAs from adult mouse spleen cells that were induced to switch to immunoglobulin A (IgA) with bacterial lipopolysaccharide (LPS) and transforming growth factor beta (TGF-beta), and identified breakpoints of S mu/S gamma 3, S mu/S gamma 2, S mu/S alpha, S gamma 3/S alpha, and S gamma 2/S alpha recombinants. The S mu recombination donor sites clustered in the 3' half of the S mu region, while the S alpha recombination acceptor sites clustered in the 5' half of the S alpha region. In addition, donor and acceptor sites of S gamma regions also clustered in the 3' and 5' parts, respectively. These site preferences are in sharp contrast to the dispersed distribution of S mu/S gamma 1 breakpoints within both S mu and S gamma 1 regions upon IgG1 switch induced by LPS and interleukin 4. Our results support the hypotheses that TGF-beta increases the frequency of switch recombination events to IgA and that the switch recombination to IgA often proceeds by successive recombination of S mu/S gamma and S gamma/S alpha.
登录
查看更多内容
影响因子：
56.9
作者：
SANGER, F
通讯作者：
SANGER, F
影响因子：
64.8
作者：
VONSCHWEDLER, U;JACK, HM;WABL, M
通讯作者：
WABL, M
影响因子：
64.8
作者：
RABBITTS, TH;FORSTER, A;BENTLEY, DL
通讯作者：
BENTLEY, DL
影响因子：
56.9
作者：
DAVIS, MM;KIM, SK;HOOD, LE
通讯作者：
HOOD, LE
影响因子：
64.5
作者：
MATSUOKA, M;YOSHIDA, K;SAKANO, H
通讯作者：
SAKANO, H