Research Note: Metabolic changes and physiological responses of broilers in the final stage of growth exposed to different environmental temperatures.
Research Note: Metabolic changes and physiological responses of broilers in the final stage of growth exposed to different environmental temperatures.
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研究注意：在暴露于不同环境温度的生长的最后阶段，肉鸡的代谢变化和生理反应。
DOI：
10.1016/j.psj.2019.11.048
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发表时间：
2020-04
期刊：
影响因子：
4.4
通讯作者：
Zhan X
中科院分区：
文献类型：
--
作者：
Wang Y;Xia L;Guo T;Heng C;Jiang L;Wang D;Wang J;Li K;Zhan X
没有关于热应激（HS）对宿主代谢谱影响的信息。本研究探讨了不同环境温度对肉仔鸡体内氧化状态、激素水平、HS指标和血浆代谢产物的影响。选用28日龄黄羽肉鸡1,680只，随机分为4组，每组6个重复。将肉鸡（29-57日龄）在饲养室（20°C、25°C、28°C和30°C）中保持连续28天。结果表明，随着温度从20 ℃升高到30 ℃，血浆皮质醇和促肾上腺皮质激素水平以及肌酸激酶和乳酸脱氢酶活性逐渐升高但胰岛素样生长因子-β水平逐渐下降。此外，热休克蛋白70在肝脏和胸肌中的表达显著增加（P < 0.01）。随着温度升高，血浆和肝脏的总抗氧化能力逐渐下降，而丙二醛水平升高。血浆谷胱甘肽过氧化物酶和肝脏总超氧化物歧化酶活性呈相似的升高趋势（P < 0.01）。此外，与25°C处理组相比，在30°C处理组中以较高（P <0.05）水平鉴定了15种代谢物，而在较低（P < 0.05）水平鉴定了2种代谢物。这些潜在的诊断生物标志物中的大多数涉及碳水化合物、氨基酸、脂质或肠道微生物组来源的代谢，表明HS影响肉鸡的代谢途径。筛选出6种候选代谢物（丙醇二酸、l-甜菜碱、酒石酸、阿洛糖、谷氨酸和新橙皮苷）作为HS诊断的生物标志物，其敏感性、特异性和准确性均达到极显著水平（P < 0.01）。总之，在生长后期，我们确定了6个血浆差异代谢物作为HS诱导的黄羽肉鸡代谢紊乱的潜在生物标志物。本研究为进一步研究HS对肉鸡代谢的影响提供了新的视角。
There is no information regarding the influence of heat stress (HS) on host metabolic profile. In this study, we investigated the effects of different environmental temperatures on oxidative status, hormone levels, HS indicators, and plasma metabolites in broilers. A total of 1,680 yellow-feather broilers (28 D old) were randomly allotted to 4 groups with 6 replicates. The broilers (29–57 D old) were maintained in thermostatic rooms (20°C, 25°C, 28°C, and 30°C) for 28 consecutive days. The results showed that the plasma cortisol and adrenocorticotropic hormone levels and creatine kinase and lactate dehydrogenase activities gradually increased when the temperature increased from 20°C to 30°C. However, the insulin-like growth factor-І level decreased gradually. Furthermore, heat shock protein 70 expression significantly increased in the liver and breast muscle (P < 0.01). As the temperature increased, the total anti-oxidant capacity in the plasma and liver gradually decreased, whereas the malondialdehyde level increased. The activity of plasma glutathione peroxidase and total superoxide dismutase in the liver showed a similar increasing trend (P < 0.01). In addition, 15 metabolites were identified at higher (P < 0.05) levels, whereas 2 metabolites were identified at lower (P < 0.05) levels in the 30°C treatment group than those in the 25°C treatment group. Most of these potentially diagnostic biomarkers are involved in carbohydrate, amino acid, lipid, or gut microbiome-derived metabolism, indicating that HS affected the metabolic pathways in broilers. Six candidate metabolites (tartronic acid, l-bethreine, tartaric acid, allose, glutaric acid, and neohesperidin) were selected as biomarkers, as they showed high sensitivity, specificity, and accuracy in diagnosing broilers under HS (P < 0.01). In conclusion, in the final stage of growth, we identified 6 plasma differential metabolites as potential biomarkers of HS-induced metabolic disorders in yellow-feathered broilers. This work offers new insights into the metabolic alterations of broilers exposed to HS and provides a new perspective for further study.
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影响因子：
4.6
作者：
Gu ZT;Li L;Wu F;Zhao P;Yang H;Liu YS;Geng Y;Zhao M;Su L
通讯作者：
Su L
DOI：
10.1152/ajpregu.00393.2005
发表时间：
2006-01-01
影响因子：
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作者：
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通讯作者：
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影响因子：
2
作者：
He, Shaoping;Li, Si;He, Jianhua
通讯作者：
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影响因子：
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作者：
Mack, L. A.;Felver-Gant, J. N.;Cheng, H. W.
通讯作者：
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