Quantitative proteomics analysis of vitreous body from type 2 diabetic patients with proliferative diabetic retinopathy.
Quantitative proteomics analysis of vitreous body from type 2 diabetic patients with proliferative diabetic retinopathy.
复制标题
2型糖尿病增生性糖尿病视网膜病变患者玻璃体的定量蛋白质组学分析
DOI：
10.1186/s12886-018-0821-3
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发表时间：
2018-06-26
影响因子：
2
通讯作者：
Lu P
中科院分区：
文献类型：
--
作者：
Li J;Lu Q;Lu P
背景：比较增殖性糖尿病视网膜病变（PDR）和特发性黄斑孔（IMH）患者玻璃体蛋白的丰度。方法对2型糖尿病合并PDR患者（n= 9）和IMH患者（n= 9）的玻璃体样本进行了基于质谱的无标记定量蛋白质组学分析，鉴定了610种蛋白的丰度。结果610个蛋白中有64个蛋白（A组）为PDR患者所特有，212个蛋白（B组）仅在IMH玻璃体中可识别。在PDR和IMH眼均可检测到的334种蛋白中，有62种蛋白与对照组相比差异显著（p< 0.05, fold change >2），其中52种蛋白（C组）和10种蛋白（D组）在PDR玻璃体中过表达和过表达。在我们的研究中，这四组中的所有蛋白都被视为重要蛋白。结论共鉴定并定量了610个蛋白，其中338个蛋白在本研究中具有重要意义。蛋白分布分析显示PDR和IMH蛋白表达明显分离。蛋白质功能分析表明，免疫和运输相关蛋白可能与PDR有关。
BackgroundTo compare the abundance of vitreous proteins between the patients with proliferative diabetic retinopathy (PDR) and idiopathic macular hole (IMH).MethodsIn this study, we performed mass spectrometry-based label-free quantitative proteomics analysis of vitreous samples from type 2 diabetic patients with PDR (n= 9) and IMH subjects (n= 9) and identified the abundance of 610 proteins.ResultsOut of 610 proteins, 64 proteins (Group A) were unique to PDR patients, while 212 proteins (Group B) could be identified in IMH vitreous only. Among the other 334 proteins that could be detected in both PDR and IMH eyes, 62 proteins differed significantly (p< 0.05, fold change > 2), which included 52 proteins (Group C) and 10 proteins (Group D) over- and under-expressed in PDR vitreous compared with the control. All proteins in these four groups were counted as significant proteins in our study.ConclusionsWe identified and quantified 610 proteins in total, which included 338 significant proteins in our study. Protein distribution analysis demonstrated a clear separation of protein expression in PDR and IMH. The protein function analysis illustrated that immunity and transport related proteins might be associated with PDR.
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影响因子：
3.7
作者：
Koss MJ;Hoffmann J;Nguyen N;Pfister M;Mischak H;Mullen W;Husi H;Rejdak R;Koch F;Jankowski J;Krueger K;Bertelmann T;Klein J;Schanstra JP;Siwy J
通讯作者：
Siwy J
糖尿病眼镜筛查：提供者和患者的知识和观点。
影响因子：
4.2
作者：
Liu Y;Swearingen R
通讯作者：
Swearingen R
影响因子：
3
作者：
Gopalakrishnan, Vidhya;Purushothaman, Parthiban;Bhaskar, Anusha
通讯作者：
Bhaskar, Anusha
影响因子：
4.4
作者：
Loukovaara, Sirpa;Nurkkala, Helka;Varjosalo, Markku
通讯作者：
Varjosalo, Markku
DOI：
10.1007/978-1-62703-392-3_1
发表时间：
2013-01-01
期刊：
Methods in molecular biology (Clifton, N.J.)
影响因子：
--
作者：
Matthiesen, Rune;Bunkenborg, Jakob
通讯作者：
Bunkenborg, Jakob