Effect of Content of Sulfate Groups in Seaweed Polysaccharides on Antioxidant Activity and Repair Effect of Subcellular Organelles in Injured HK-2 Cells.
Effect of Content of Sulfate Groups in Seaweed Polysaccharides on Antioxidant Activity and Repair Effect of Subcellular Organelles in Injured HK-2 Cells.
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DOI：
10.1155/2017/2542950
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发表时间：
2017
影响因子：
--
通讯作者：
Ouyang JM
中科院分区：
文献类型：
--
作者：
Ma XT;Sun XY;Yu K;Gui BS;Gui Q;Ouyang JM
研究了条斑紫菜、龙须菜、羊栖菜和裙带菜4种降解海藻多糖对HK-2细胞亚细胞结构损伤的修复作用。四种DSP具有相似的分子量，但具有不同含量的硫酸根基团（即，17.9%、13.3%、8.2%和5.5%）。用2.8mmol/L草酸盐建立HK-2细胞损伤模型，用60 μg/mL的各种DSP进行修复。随着DSP中硫酸基含量的增加，自由基的清除活性和还原能力均提高。四种DSP对损伤的HK-2细胞亚细胞器均有修复作用。经DSP修复后，乳酸脱氢酶释放量减少，细胞膜和溶酶体完整性增加，Δ λ m增加，G1期阻滞细胞受到抑制，S期比例增加，细胞凋亡率和坏死率明显降低。硫酸基含量越高，多糖的修复能力越强。这些多糖，特别是硫酸基含量较高的多糖，可能成为防治肾结石的潜在药物。
This study aims to investigate the repair effect of subcellular structure injuries of the HK-2 cells of four degraded seaweed polysaccharides (DSPs), namely, the degraded Porphyra yezoensis, Gracilaria lemaneiformis, Sargassum fusiform, and Undaria pinnatifida polysaccharides. The four DSPs have similar molecular weight, but with different content of sulfate groups (i.e., 17.9%, 13.3%, 8.2%, and 5.5%, resp.). The damaged model was established using 2.8 mmol/L oxalate to injure HK-2 cells, and 60 μg/mL of various DSPs was used to repair the damaged cells. With the increase of sulfate group content in DSPs, the scavenging activity of radicals and their reducing power were all improved. Four kinds of DSPs have repair effect on the subcellular organelles of damaged HK-2 cells. After being repaired by DSPs, the release amount of lactate dehydrogenase was decreased, the integrity of cell membrane and lysosome increased, the Δψm increased, the cell of G1 phase arrest was inhibited, the proportion of S phase increased, and cell apoptotic and necrosis rates were significantly reduced. The greater the content of sulfate group is, the stronger is the repair ability of the polysaccharide. These DSPs, particularly the polysaccharide with higher sulfate group content, may be a potential drug for the prevention and cure of kidney stones.
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影响因子：
3.3
作者：
Imbs, Tatiana I.;Skriptsova, Anna V.;Zvyagintseva, Tatiana N.
通讯作者：
Zvyagintseva, Tatiana N.
影响因子：
3.7
作者：
Roy Chowdhury S;Sengupta S;Biswas S;Sinha TK;Sen R;Basak RK;Adhikari B;Bhattacharyya A
通讯作者：
Bhattacharyya A
枸杞多糖通过ROS-NO途径对6-OHDA诱导的PC12细胞凋亡的保护作用。
DOI：
10.3390/molecules20010293
发表时间：
2014-12-24
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
Gao K;Liu M;Cao J;Yao M;Lu Y;Li J;Zhu X;Yang Z;Wen A
通讯作者：
Wen A
影响因子：
11.2
作者：
Geng, Zhao-Hua;Huang, Lan;Song, Yao-Ming
通讯作者：
Song, Yao-Ming
影响因子：
3.6
作者：
Garcia-Casal, Maria N.;Ramirez, Jose;Quiroga, Maria F.
通讯作者：
Quiroga, Maria F.