Nuclear, mitochondrial and plastid gene phylogenies of Dinophysis miles (Dinophyceae): evidence of variable types of chloroplasts.
Nuclear, mitochondrial and plastid gene phylogenies of Dinophysis miles (Dinophyceae): evidence of variable types of chloroplasts.
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DOI：
10.1371/journal.pone.0029398
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Lin S
中科院分区：
文献类型：
--
作者：
Qiu D;Huang L;Liu S;Lin S
甲藻属是海洋甲藻中具有重要生态和进化意义的类群，但其分子系统发育位置和生态特征如营养模式等仍知之甚少。在这里，一个人口的Dinophysis英里变种。2010年3月从中国南海海域采集了籼稻样品。PCR扩增核核糖体RNA（rDNA）基因SSU、ITS 1 -5. 8 S-ITS 2和LSU，线粒体编码细胞色素B（co B）和细胞色素C氧化酶亚基I（cox 1）的基因，以及质体rDNA SSU。基于cob、cox 1和核rRNA区域的系统发育分析表明，D. miles与D. tripos和D.尾状体而与D.渐尖的沿着形态学和ITS 1 -5. 8 S-ITS 2分子数据证实该群体为D.迈尔斯湾indica。ITS 1 -5. 8 S-ITS 2片段是区分D.远离其他甲藻物种三种不同类型的质体rDNA序列被检测到，分别属于一个隐藻，一个附着菌，和一个蓝藻的质体。这是第一个文件的三个光合实体与甲藻种。而蓝细菌序列可能代表了D. miles细胞中，对隐芽植物和附着植物质体序列的检测表明，D.迈尔斯可能保留了一种以上类型的质体从它的猎物藻类暂时用于光合作用。这一结果，连同最近的研究结果的质体类型在其他甲藻物种，表明需要更系统的研究，以了解复杂的营养生理的甲藻属。
The Dinophysis genus is an ecologically and evolutionarily important group of marine dinoflagellates, yet their molecular phylogenetic positions and ecological characteristics such as trophic modes remain poorly understood. Here, a population of Dinophysis miles var. indica was sampled from South China Sea in March 2010. Nuclear ribosomal RNA gene (rDNA) SSU, ITS1-5.8S-ITS2 and LSU, mitochondrial genes encoding cytochrome B (cob) and cytochrome C oxidase subunit I (cox1), and plastid rDNA SSU were PCR amplified and sequenced. Phylogenetic analyses based on cob, cox1, and the nuclear rRNA regions showed that D. miles was closely related to D. tripos and D. caudata while distinct from D. acuminata. Along with morphology the LSU and ITS1-5.8S-ITS2 molecular data confirmed that this population was D. miles var. indica. Furthermore, the result demonstrated that ITS1-5.8S-ITS2 fragment was the most effective region to distinguish D. miles from other Dinophysis species. Three distinct types of plastid rDNA sequences were detected, belonging to plastids of a cryptophyte, a haptophyte, and a cyanobacterium, respectively. This is the first documentation of three photosynthetic entities associated with a Dinophysis species. While the cyanobacterial sequence likely represented an ectosymbiont of the D. miles cells, the detection of the cryptophyte and haptophyte plastid sequences indicates that the natural assemblage of D. miles likely retain more than one type of plastids from its prey algae for temporary use in photosynthesis. The result, together with recent findings of plastid types in other Dinophysis species, suggests that more systematic research is required to understand the complex nutritional physiology of this genus of dinoflagellates.
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影响因子：
11
作者：
Muhling, Martin;Woolven-Allen, John;Joint, Ian
通讯作者：
Joint, Ian
影响因子：
2.9
作者：
Handy, Sara M.;Bachvaroff, Tsvetan R.;Delwiche, Charles F.
通讯作者：
Delwiche, Charles F.
影响因子：
2.9
作者：
Jensen, Maria Hastrup;Daugbjerg, Niels
通讯作者：
Daugbjerg, Niels
DOI：
10.1080/00785326.1991.10429712
发表时间：
1991-06-01
期刊：
OPHELIA
影响因子：
--
作者：
LUCAS, IAN
通讯作者：
LUCAS, IAN
影响因子：
1.9
作者：
Marasigan, AN;Sato, S;Kodama, M
通讯作者：
Kodama, M